Dasilva M, Grieve K L, Cudeiro J, Rivadulla C
Neuroscience and Motor Control Laboratory (Neurocom), Department of Medicine-INEF, University of A Coruña, Spain; Institute of Biomedical Research of A Coruña (INIBIC), Spain.
Faculty of Life Sciences, University of Manchester, UK.
Neuroscience. 2014 Apr 18;265:72-82. doi: 10.1016/j.neuroscience.2014.01.049. Epub 2014 Feb 5.
The endocannabinoid system is a modulatory system that has been strongly associated with the regulation of functions as learning and memory, pain perception and sensory physiology in many areas of the central nervous system. However, although a role in sensory processing has been demonstrated at the level of the thalamus, the influence of the endocannabinoid system on thalamic rhythms and oscillations has been less studied, despite the fact that such activities are significant characteristics of the thalamic state. The present work aimed to characterize the role of anandamide (AEA) - one of the endogenous CB1 receptor agonists - and AM251 - a CB1 antagonist - in the modulation of burst firing and oscillatory activity present in the dLGN of the anesthetized rat. Administration of AEA (0.5mg/kg iv) increased the number of bursts in the majority of the cells tested and induced the appearance of a slow delta-like (1.5Hz) oscillatory activity. These effects were CB1-mediated, as demonstrated by the complete antagonism during the co-application of AM251 (0.5mg/kg iv). Thus, by demonstrating that the AEA-mediated activation of CB1 receptors increases spontaneous bursting and oscillatory activity in the thalamus our study infers that endocannabinoids could have a role in processes controlling the sleep-wake cycle and level of arousal.
内源性大麻素系统是一种调节系统，在中枢神经系统的许多区域，它与学习和记忆、痛觉感知及感觉生理学等功能的调节密切相关。然而，尽管已证实在丘脑水平内源性大麻素系统在感觉处理中发挥作用，但该系统对丘脑节律和振荡的影响却鲜有研究，尽管这些活动是丘脑状态的重要特征。本研究旨在表征花生四烯乙醇胺（AEA）——内源性CB1受体激动剂之一——和AM251——一种CB1拮抗剂——在调节麻醉大鼠背外侧膝状体（dLGN）中爆发式放电和振荡活动方面的作用。静脉注射AEA（0.5mg/kg）可增加大多数受试细胞的爆发次数，并诱导出现类似慢波δ（1.5Hz）的振荡活动。如静脉注射AM251（0.5mg/kg）共同给药时的完全拮抗作用所示，这些效应是由CB1介导的。因此，通过证明AEA介导的CB1受体激活可增加丘脑中的自发爆发式放电和振荡活动，我们的研究推断内源性大麻素可能在控制睡眠-觉醒周期和觉醒水平的过程中发挥作用。
