Marugg J D, Nielander H B, Horrevoets A J, van Megen I, van Genderen I, Weisbeek P J
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
J Bacteriol. 1988 Apr;170(4):1812-9. doi: 10.1128/jb.170.4.1812-1819.1988.
In iron-limited environments, the plant-growth-stimulating Pseudomonas putida WCS358 produces a yellow-green fluorescent siderophore called pseudobactin 358. The transcriptional organization and the iron-regulated expression of a major gene cluster involved in the biosynthesis and transport of pseudobactin 358 were analyzed in detail. The cluster comprises a region with a minimum length of 33.5 kilobases and contains at least five transcriptional units, of which some are relatively large. The directions of transcription of four transcriptional units were determined by RNA-RNA hybridization and by analysis in Escherichia coli minicells. The latter also demonstrated that large polypeptides were encoded by these transcriptional units. The results allowed us to localize several promoter regions on the DNA. The iron-dependent expression of at least two genes within this cluster appears to be regulated at the transcriptional level.
在铁限制环境中，促进植物生长的恶臭假单胞菌WCS358产生一种黄绿荧光铁载体，称为假铁载体358。详细分析了参与假铁载体358生物合成和转运的一个主要基因簇的转录组织和铁调节表达。该基因簇包含一个最小长度为33.5千碱基的区域，至少包含五个转录单元，其中一些相对较大。通过RNA-RNA杂交和在大肠杆菌微小细胞中的分析确定了四个转录单元的转录方向。后者还表明这些转录单元编码大的多肽。这些结果使我们能够在DNA上定位几个启动子区域。该基因簇中至少两个基因的铁依赖性表达似乎在转录水平上受到调节。
