Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, United States; Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT 59717, United States; Chemical and Biological Signature Science, Pacific Northwest National Laboratories, Richland, WA 99352, United States.
Department of Biological Engineering, Utah State University, Logan, UT 84322, United States.
Bioresour Technol. 2014 Mar;156:206-15. doi: 10.1016/j.biortech.2014.01.001. Epub 2014 Jan 10.
Microalgal biofilm based technologies are of keen interest due to their high biomass concentrations and ability to utilize light and CO2. While photoautotrophic biofilms have long been used for wastewater remediation, biofuel production represents a relatively new and under-represented focus area. However, the direct measurement and characterization of fundamental parameters required for industrial control are challenging due to biofilm heterogeneity. This study evaluated oxygenic photosynthesis and respiration on two distinct microalgal biofilms cultured using a novel rotating algal biofilm reactor operated at field- and laboratory-scales. Clear differences in oxygenic photosynthesis and respiration were observed based on different culturing conditions, microalgal composition, light intensity and nitrogen availability. The cultures were also evaluated as potential biofuel synthesis strategies. Nitrogen depletion was not found to have the same effect on lipid accumulation compared to traditional planktonic microalgal studies. Physiological characterizations of these microalgal biofilms identify fundamental parameters needed to understand and control process optimization.
基于微藻生物膜的技术因其高生物质浓度以及利用光和 CO2 的能力而备受关注。虽然光自养生物膜早已被用于废水修复，但生物燃料生产是一个相对较新且研究不足的重点领域。然而，由于生物膜的异质性，对于工业控制所需的基本参数的直接测量和表征具有挑战性。本研究使用新型旋转藻生物膜反应器在现场和实验室规模上培养了两种不同的微藻生物膜，评估了好氧光合作用和呼吸作用。根据不同的培养条件、微藻组成、光照强度和氮供应，观察到好氧光合作用和呼吸作用存在明显差异。这些培养物还被评估为潜在的生物燃料合成策略。与传统的浮游微藻研究相比，氮的消耗对脂质积累的影响并不相同。对这些微藻生物膜的生理特性进行表征，可确定理解和控制工艺优化所需的基本参数。
