Prykhozhij Sergey V, Berman Jason N
Department of Pediatrics, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
Department of Pediatrics, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
Dev Comp Immunol. 2014 Sep;46(1):74-83. doi: 10.1016/j.dci.2014.01.023. Epub 2014 Feb 6.
Immunological and hematological research using the zebrafish (Danio rerio) has significantly advanced our understanding of blood lineage ontology, cellular functions and mechanisms, and provided opportunities for disease modeling. Mast cells are an immunological cell type involved in innate and adaptive immune systems, hypersensitivity reactions and cancer progression. The application of zebrafish to study mast cell biology exploits the developmental and imaging opportunities inherent in this model system to enable detailed genetic and molecular studies of this lineage outside of traditional mammalian models. In this review, we first place the importance of mast cell research in zebrafish into the context of comparative studies of mast cells in other fish species and highlight its advantages due to superior experimental tractability and direct visualization in transparent embryos. We discuss current and future tools for mast cell research in zebrafish and the notable results of using zebrafish for understanding mast cell fate determination and our development of a systemic mastocytosis model.
利用斑马鱼（Danio rerio）进行的免疫学和血液学研究极大地推进了我们对血液谱系本体论、细胞功能和机制的理解，并为疾病建模提供了机会。肥大细胞是一种参与先天和适应性免疫系统、超敏反应和癌症进展的免疫细胞类型。将斑马鱼应用于肥大细胞生物学研究利用了该模型系统固有的发育和成像机会，从而能够在传统哺乳动物模型之外对这一细胞谱系进行详细的遗传和分子研究。在这篇综述中，我们首先将斑马鱼肥大细胞研究的重要性置于对其他鱼类物种肥大细胞的比较研究背景中，并强调由于其卓越的实验可操作性以及在透明胚胎中的直接可视化而具有的优势。我们讨论了斑马鱼肥大细胞研究的当前和未来工具，以及利用斑马鱼理解肥大细胞命运决定的显著成果，还有我们对系统性肥大细胞增多症模型的开发。
