γ-氨基丁酸A型受体印防己毒素位点的特性
Characterization of the picrotoxin site of GABAA receptors.
作者信息
Mehta Ashok K, Ticku Maharaj K
机构信息
The University of Texas Health Science Center, San Antonio, Texas.
出版信息
Curr Protoc Pharmacol. 2013 Dec 2;63:1.18.1-1.18.18. doi: 10.1002/0471141755.ph0118s63.
PMID:24510752
Abstract
This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands. Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included.
摘要
本单元描述了一种使用各种放射性配体对大鼠脑膜中GABAA受体印防己毒素位点进行表征的体外测定方法。其中包括用于Scatchard分析（Kd、Bmax测定）、结合动力学、解离动力学和竞争测定（IC50、Ki测定）的方法及代表性数据。
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