叶酸对GABAa受体复合物中印防己毒素敏感位点的影响。

The influence of folic acid on the picrotoxin-sensitive site of the GABAa-receptor complex.

作者信息

Van Rijn C M, Van der Velden T J, Rodrigues de Miranda J F, Feenstra M G, Hommes O R

机构信息

Dept. of Experimental Neurology, University of Nijmegen, The Netherlands.

出版信息

Epilepsy Res. 1988 May-Jun;2(3):215-8. doi: 10.1016/0920-1211(88)90058-7.

DOI:10.1016/0920-1211(88)90058-7

PMID:2848698

Abstract

The site of action responsible for the convulsive effect of folic acid was investigated in vitro. Folic acid (ECmax 5 x 10(-4) M) enhances the binding of the cage convulsant [3H]t-butylbicycloorthobenzoate ([3H]TBOB) to rat brain membranes, namely to 130% of control in the absence of GABA and to over 300% of control in the presence of physiological concentrations of GABA. Analysis of the binding parameters reveals that folic acid increases the apparent number of [3H]TBOB binding sites.

摘要

在体外研究了负责叶酸惊厥作用的作用位点。叶酸（最大效应浓度5×10⁻⁴ M）增强了笼状惊厥剂[³H]叔丁基双环邻苯二甲酸酯（[³H]TBOB）与大鼠脑膜的结合，即在无γ-氨基丁酸（GABA）时为对照的130%，在生理浓度的GABA存在时为对照的300%以上。结合参数分析表明，叶酸增加了[³H]TBOB结合位点的表观数量。