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胁迫指数在水稻基因型低温和深播胁迫筛选中的应用。

Application of stress indices for low temperature and deep sowing stress screening of rice genotypes.

作者信息

Bevilacqua Caroline Borges, Monzon Daisy Ramirez, Venske Eduardo, Basu Supratim, Zimmer Paulo Dejalma

机构信息

Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Dep. Fitotecnia, Programa de Pós-graduação em Ciência e Tecnologia de Sementes, Caixa Postal 354, CEP 96.001-970, Pelotas, RS, Brasil.

115 Plant Sciences Building, University of Arkansas, Fayetteville, AR-72701, USA.

出版信息

Pak J Biol Sci. 2013 Nov 15;16(22):1618-22. doi: 10.3923/pjbs.2013.1618.1622.

DOI:10.3923/pjbs.2013.1618.1622
PMID:24511713
Abstract

Low temperature or cold stress and deep sowing plays a pivotal role in limiting rice (Oryza sativa L.) productivity in the temperate rice growing regions as well as in tropical high lands worldwide. A better understanding of stress tolerance mechanism in rice plants will help to develop rice germplasm with improved field level tolerance under variable temperature and sowing deep conditions. Using previously developed stress indices, this study presents results from low temperature and deep sowing screening of four rice genotypes. A group of 25 seeds per replicate (total of 3 replicates) was subjected to stress by deep sowing (15 cm) while another group was subjected to cold stress (13 degrees C-10 h/18 degrees C-14 h) and the control group remained under optimum conditions (25 degrees C and sowing depth of 1.5 cm). The Geometric Mean (GM), Stress Tolerance Index (STI) and Stress Susceptibility Index (SSI) were used to evaluate the genotypic performance under control and stress conditions. The results indicate that it was possible to identify superior genotypes for tolerance based on their stress indices. The indices although correlated, were found to be effective for the selection of genotypes with good yield potential under control and stress treatments and can now be used for genotypic screening under field conditions.

摘要

低温或冷胁迫以及深播在限制温带水稻种植区以及全球热带高地的水稻(Oryza sativa L.)生产力方面起着关键作用。更好地了解水稻植株的胁迫耐受机制将有助于培育出在可变温度和播种深度条件下具有更高田间耐受性的水稻种质。利用先前开发的胁迫指数,本研究展示了对四种水稻基因型进行低温和深播筛选的结果。每个重复组有25粒种子(共3个重复组),一组通过深播(15厘米)施加胁迫,另一组遭受冷胁迫(13摄氏度 - 10小时/18摄氏度 - 14小时),对照组保持在最佳条件下(25摄氏度和1.5厘米的播种深度)。几何平均数(GM)、胁迫耐受指数(STI)和胁迫敏感指数(SSI)用于评估对照和胁迫条件下的基因型表现。结果表明,基于胁迫指数有可能鉴定出耐受性优良的基因型。这些指数虽然相关,但被发现对于选择在对照和胁迫处理下具有良好产量潜力的基因型是有效的,现在可用于田间条件下的基因型筛选。

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