Dowling J P, Mathews D C
Department of Tissue Pathology, Prince Henry's Hospital.
Pathology. 1987 Oct;19(4):410-4. doi: 10.3109/00313028709103893.
The application of silver methenamine impregnation (SMI) to renal electronmicroscopy (EM) has been relatively neglected, but the technique has proved to be a useful aid in renal biopsy diagnosis. Tissues for SMI were fixed in glutaraldehyde/osmium and were embedded in either L.R. White resin or Epon. En-bloc staining of tissue with uranyl acetate was undertaken, but this procedure is considered optional. In our hands the technique has been valuable in the rapid assessment of renal ultrastructure in "routine" biopsies and especially in the diagnosis of complex or subtle changes in a variety of subtypes of glomerulonephritis, including the early membranous, familial-Alport type, mesangiocapillary and difficult to classify categories.
六胺银浸渍法（SMI）在肾脏电子显微镜检查（EM）中的应用相对被忽视，但该技术已被证明对肾脏活检诊断有很大帮助。用于SMI的组织用戊二醛/锇固定，然后包埋在低粘度LR White树脂或环氧树脂中。组织用醋酸铀进行整体染色，但该步骤被认为是可选的。在我们的实践中，该技术对于“常规”活检中肾脏超微结构的快速评估非常有价值，特别是在诊断各种类型肾小球肾炎的复杂或细微变化方面，包括早期膜性、家族性阿尔波特型、系膜毛细血管性以及难以分类的类型。
