Reed B V
Department of Physical Therapy, School of Allied Health Sciences, University of Vermont, Burlington 05405.
Phys Ther. 1988 Apr;68(4):491-5. doi: 10.1093/ptj/68.4.491.
The purpose of this study was to determine whether high voltage pulsed electrical stimulation reduces microvascular permeability to plasma proteins in a simulation of acute edema. Fourteen male golden hamsters were anesthetized and prepared for fluorescence microscopy of the cheek pouch. Intravenous fluorescein-labeled dextran (MW 150,000) served as a tracer of plasma proteins. Protein leaks from the microvessels were quantified every 5 minutes for a 25-minute baseline period and again after a 5-minute superfusion with 10(-5)-M histamine. High voltage stimulation (HVS) was applied simultaneously with the histamine to the vascular bed of the treatment animals in a dosage of 10, 30, or 50 V; the control animals received no electrical stimulation. Histamine increased microvessel leakiness in all groups, but the number of posthistamine microvessel leaks was significantly less in animals that received a 30- or 50-V dose of HVS than in the control animals or those that received a 10-V dose. The study results suggest that at intensities greater than a threshold dose, HVS reduces microvessel leakiness. Reduced microvessel leakiness may be one mechanism by which HVS retards edema formation.
本研究的目的是在急性水肿模拟实验中，确定高压脉冲电刺激是否会降低微血管对血浆蛋白的通透性。14只雄性金黄地鼠被麻醉，并准备用于颊囊的荧光显微镜检查。静脉注射荧光素标记的葡聚糖（分子量150,000）作为血浆蛋白的示踪剂。在25分钟的基线期内，每5分钟对微血管中的蛋白质渗漏进行定量，在与10（-5）-M组胺进行5分钟的灌注后再次进行定量。在给予治疗动物的血管床组胺的同时，以10、30或50 V的剂量施加高压刺激（HVS）；对照动物不接受电刺激。组胺增加了所有组的微血管渗漏，但接受30或50 V HVS剂量的动物在组胺刺激后的微血管渗漏数量明显少于对照动物或接受10 V剂量的动物。研究结果表明，在高于阈值剂量的强度下，HVS会降低微血管渗漏。微血管渗漏减少可能是HVS延缓水肿形成的一种机制。
