Hu Jian, Feng Xiangping, Yang Zhongguo, Chen Zhuoxin, Zhang Wenqing
State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
Dev Comp Immunol. 2014 Jul;45(1):10-20. doi: 10.1016/j.dci.2014.01.025. Epub 2014 Feb 7.
A continuous cell line, SYSU-OfHe-C, from larval hemocytes of corn borer, Ostrinia furnacalis was established. With increasing passages, the cells grew increasingly faster, and approximately 45% of the cells were in division at passage 55. The culture was mainly composed of two types of cells, granulocytes and plasmatocytes, which showed different division and proliferation behaviors, but possessed similar phagocytic ability. Its spreading ability was significantly weaker than that of hemocytes from naïve larva; however, it could be promoted by larval plasma. Furthermore, its encapsulation ability was also promoted by larval plasma to form multilayer capsules on Sephadex A-25 beads. Finally, the expression of several immune-related genes was verified after provocation by microbes or Sephadex beads. These results indicated that the cell line possessed immune ability depending on the presence of plasma of naïve larvae and are beneficial to studies of insect cellular systems.
从玉米螟（Ostrinia furnacalis）幼虫血细胞中建立了一个连续细胞系SYSU-OfHe-C。随着传代次数的增加，细胞生长越来越快，在第55代时约45%的细胞处于分裂状态。该培养物主要由两种类型的细胞组成，即粒细胞和浆细胞，它们表现出不同的分裂和增殖行为，但具有相似的吞噬能力。其铺展能力明显弱于未处理幼虫的血细胞；然而，幼虫血浆可促进其铺展。此外，幼虫血浆也可促进其包囊能力，使其在葡聚糖A-25珠上形成多层包囊。最后，在受到微生物或葡聚糖珠刺激后，验证了几个免疫相关基因的表达。这些结果表明，该细胞系具有依赖于未处理幼虫血浆的免疫能力，有利于昆虫细胞系统的研究。
