Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 N. University St., Peoria, IL 61604, USA.
Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 N. University St., Peoria, IL 61604, USA.
Carbohydr Res. 2014 Mar 31;387:1-3. doi: 10.1016/j.carres.2014.01.013. Epub 2014 Jan 23.
The main acceptor product of glucansucrases with d-mannose has not previously been identified. We used glucansucrases that form water-insoluble α-d-glucans to produce increased yields of acceptor products from d-mannose, and identified the major product as 6-O-α-d-glucopyranosyl-d-mannose. Glucansucrases that synthesize insoluble α-d-glucans produced higher yields of the disaccharide compared to typical dextransucrases.
具有 d-甘露糖的葡聚糖蔗糖酶的主要接受产物以前尚未被鉴定。我们使用形成不溶于水的α-d-葡聚糖的葡聚糖蔗糖酶来增加 d-甘露糖的接受产物的产量,并将主要产物鉴定为 6-O-α-d-吡喃葡萄糖基-d-甘露糖。与典型的右旋糖酐蔗糖酶相比,合成不溶性α-d-葡聚糖的葡聚糖蔗糖酶产生更高产量的二糖。