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肠系膜明串珠菌YTU 40的葡聚糖蔗糖酶40产生的支链葡聚糖和乙酰化低聚葡萄糖的表征及免疫调节作用

Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40.

作者信息

Dere Sevda, İspirli Hümeyra, Korkmaz Kader, Tuncil Yunus Emre, Sagdic Osman, Narbad Arjan, Ayyash Mutamed, Dertli Enes

机构信息

Chemical and Metallurgical Engineering Faculty, Food Engineering Department, Yildiz Technical University, Istanbul, Turkey.

Engineering Faculty, Food Engineering Department, Bayburt University, Bayburt, Turkey.

出版信息

Sci Rep. 2025 Apr 3;15(1):11412. doi: 10.1038/s41598-025-96324-9.

DOI:10.1038/s41598-025-96324-9
PMID:40181138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11968854/
Abstract

Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected byH andC NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.

摘要

葡聚糖蔗糖酶（GS）是用于生产具有潜在生物活性特征的不同葡聚糖和低聚葡萄糖结构的功能性酶。在本研究中，在肠系膜明串珠菌YTU 40的基因组中鉴定出一种活性葡聚糖蔗糖酶（GS40），并对其进行了进一步克隆、异源表达和生化表征。通过氢核磁共振和碳核磁共振分析检测到，GS40能够产生一种含有约20%（1→3）-α-D-葡萄糖残基的支链葡聚糖。进行了GS40与麦芽糖和纤维二糖的受体反应，并通过薄层色谱、核磁共振和液相色谱-质谱分析检测到产生了不同的麦芽糖/纤维二糖衍生物乙酰化低聚葡萄糖。通过测定支链葡聚糖和低聚葡萄糖在触发白细胞介素-12、白细胞介素-10、白细胞介素-4和肿瘤坏死因子-α细胞因子水平方面的免疫调节作用，对它们的生物活性特征进行了测试。这些发现对于揭示葡聚糖蔗糖酶在生产可用作食品和其他行业功能成分的低聚葡萄糖方面的作用可能具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/14aa7bfc224d/41598_2025_96324_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/35d4df2abb73/41598_2025_96324_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/9f551bb46474/41598_2025_96324_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/e774cfc9e880/41598_2025_96324_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/fcc0e1617477/41598_2025_96324_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/3ff53d628ff8/41598_2025_96324_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/14aa7bfc224d/41598_2025_96324_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/35d4df2abb73/41598_2025_96324_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/9f551bb46474/41598_2025_96324_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/e774cfc9e880/41598_2025_96324_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/fcc0e1617477/41598_2025_96324_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/3ff53d628ff8/41598_2025_96324_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b01d/11968854/14aa7bfc224d/41598_2025_96324_Fig6_HTML.jpg

相似文献

[1]
Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40.

Sci Rep. 2025-4-3

[2]
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[3]
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[4]
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[5]
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[6]
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[7]
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[8]
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[9]
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[10]
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本文引用的文献

[1]
Metatranscriptomic analysis indicates prebiotic effect of isomalto/malto-polysaccharides on human colonic microbiota in-vitro.

Sci Rep. 2024-8-14

[2]
Acetylation at the O-6 position of t-Glc improved immunoactivity of α-1,6-glucan from longan by additionally activating Dectin-1 and CD14 receptors.

Carbohydr Polym. 2023-11-15

[3]
Utilisation of an active branching sucrase from Lactobacillus kunkeei AP-37 to produce techno-functional poly-oligosaccharides.

Int J Biol Macromol. 2023-5-1

[4]
Structural characterization and biological evaluation of a new O-acetyl-1,4-linked-β-d-mannan possessed potential application in hydrophilic polymer materials from Dendrobium devonianum.

Int J Biol Macromol. 2022-7-31

[5]
Bioactive and technological properties of an α-D-glucan synthesized by Weissella cibaria PDER21.

Carbohydr Polym. 2022-6-1

[6]
Isolation, characterization and comparative genomics of potentially probiotic Lactiplantibacillus plantarum strains from Indian foods.

Sci Rep. 2022-2-4

[7]
Structural and bioactive characteristics of a dextran produced by Lactobacillus kunkeei AK1.

Int J Biol Macromol. 2022-3-1

[8]
Fructooligosaccharides on inflammation, immunomodulation, oxidative stress, and gut immune response: a systematic review.

Nutr Rev. 2022-3-10

[9]
Reuteransucrase-catalytic kinetic modeling and functional characteristics for novel prebiotic gluco-oligomers.

Food Funct. 2020-8-19

[10]
Synthesis of glucooligosaccharides with prebiotic potential by glucansucrase URE 13-300 acceptor reactions with maltose, raffinose and lactose.

Eng Life Sci. 2018-5-22

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