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在阿根廷首次从人胎盘中分离出刚地弓形虫并进行分子特征分析。

First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina.

作者信息

Pardini Lais, Carral Liliana A, Bernstein Mariana, Gos María L, Olejnik Patricia, Unzaga Juan M, Kaufer Federico J, Freuler Cristina B, Durlach Ricardo A, Venturini María C

机构信息

Laboratorio de Inmunoparasitología, FCV, UNLP, Argentina; CONICET, Argentina.

Centro de toxoplasmosis y otras zoonosis, Hospital Alemán, Buenos Aires, Argentina.

出版信息

Parasitol Int. 2014 Apr;63(2):470-2. doi: 10.1016/j.parint.2013.10.011. Epub 2013 Oct 26.

DOI:10.1016/j.parint.2013.10.011
PMID:24513795
Abstract

Blood sample and placenta were taken from a 37-week pregnant woman; serologic results indicated acute toxoplasmosis. Placenta was inoculated into mice. Seropositive mice were sacrificed and tissue cysts from brain were inoculated into new mice. Specific DNA was detected by PCR, and the isolate was characterized as Type II by nPCR-RFLP for nSAG2, SAG3, BTUB, GRA6, c29-2, c22-8, L358, PK1 and Apico markers. This is the first isolation and molecular characterization of Toxoplasma gondii from humans in Argentina.

摘要

从一名怀孕37周的妇女身上采集了血样和胎盘；血清学结果显示为急性弓形虫病。将胎盘接种到小鼠体内。对血清反应呈阳性的小鼠实施安乐死，并将其脑组织囊肿接种到新的小鼠体内。通过聚合酶链反应检测特异性DNA，并通过针对nSAG2、SAG3、BTUB、GRA6、c29-2、c22-8、L358、PK1和Apico标记物的巢式聚合酶链反应-限制性片段长度多态性分析，将该分离株鉴定为II型。这是在阿根廷首次从人体分离出刚地弓形虫并进行分子特征分析。

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引用本文的文献

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Is Toxoplasma gondii type related to clinical outcome in human congenital infection? Systematic and critical review.弓形虫类型与人类先天性感染的临床结局有关吗？系统评价与批判性综述。
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