Hermans Pim, Adams Hendrik, Detmers Frank
BAC BV, Life Technologies, Leiden, The Netherlands.
Methods Mol Biol. 2014;1131:297-314. doi: 10.1007/978-1-62703-992-5_19.
Ever since the introduction of bacterial derived surface proteins like protein A that demonstrate a natural binding reactivity towards antibodies, affinity chromatography has evolved into a well-established technology for the purification of antibodies and antibody fragments. Although high selectivity is provided by these types of affinity ligands, not all antibodies or antibody fragments are covered, which then forces the use of non-affinity-based processes that are less selective and often result in lower one-step purity and yield. To fill these gaps, we here describe a novel range of CaptureSelect(™) affinity resins that enables immunoaffinity chromatography for a much broader range of antibody targets.
自从引入像蛋白A这样的细菌衍生表面蛋白以来，亲和色谱技术已发展成为一种成熟的抗体和抗体片段纯化技术，蛋白A对抗体具有天然的结合反应性。尽管这些类型的亲和配体具有高选择性，但并非所有抗体或抗体片段都适用，这就迫使人们使用选择性较低的非亲和方法，而这些方法往往导致一步纯化的纯度和产率较低。为了填补这些空白，我们在此描述了一系列新型的CaptureSelect(™)亲和树脂，这些树脂可实现针对更广泛抗体靶点的免疫亲和色谱。
