Baldini A, Felli M P, Ravenna L, Camaioni A, de Capoa A, Spadoni G L, Cianfarani S, Boscherini B
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
Cytogenet Cell Genet. 1988;47(1-2):22-5. doi: 10.1159/000132498.
Differential activity of rRNA gene clusters following growth-hormone administration has been demonstrated in cultured lymphocytes from subjects with different genetic backgrounds, i.e., with or without in vivo peripheral responsiveness to the hormone. The influence of different culture conditions on ribosomal gene responsiveness was also tested. Ribosomal gene activity was evaluated by selective silver staining of nucleolus organizing regions. The results show that hormone-induced enhancement of transcriptional activity requires both genetically determined cell responsiveness and environmentally determined permissive factors.
生长激素给药后，rRNA基因簇在来自不同遗传背景（即体内对该激素有或无外周反应性）的受试者的培养淋巴细胞中表现出不同的活性。还测试了不同培养条件对核糖体基因反应性的影响。通过对核仁组织区进行选择性银染来评估核糖体基因活性。结果表明，激素诱导的转录活性增强既需要基因决定的细胞反应性，也需要环境决定的许可因子。
