Niederwieser D, Auböck J, Troppmair J, Herold M, Schuler G, Boeck G, Lotz J, Fritsch P, Huber C
Department of Internal Medicine, University of Innsbruck, Austria.
J Immunol. 1988 Apr 15;140(8):2556-64.
The MHC Ag expression on the surface of keratinocytes is altered after treatment with IFN. IFN-gamma induces, as expected, a strong increase in class I MHC Ag expression as well as de novo expression of class II MHC Ag, whereas IFN-alpha 2 only slightly increases class I MHC Ag and does not induce keratinocytes to express class II MHC Ag. We used untreated and IFN-pretreated keratinocytes as stimulators and also as targets to study whether IFN-induced MHC Ag changes would alter the immunogenicity of keratinocytes in alloimmune responses. It was found that class II MHC Ag-carrying keratinocytes were unable to induce the proliferation of resting lymphocytes, but did stimulate T blasts. Untreated keratinocytes were virtually resistant to the lysis by classical CTL but became susceptible after exposure to IFN-gamma, but not IFN-alpha 2 at physiologic doses. These data demonstrate mechanisms by which the release of IFN-gamma might contribute to the development of disease such as the graft vs host disease.
用干扰素处理后，角质形成细胞表面的主要组织相容性复合体（MHC）抗原表达会发生改变。正如预期的那样，γ干扰素可诱导I类MHC抗原表达显著增加以及II类MHC抗原的从头表达，而α2干扰素仅略微增加I类MHC抗原，且不会诱导角质形成细胞表达II类MHC抗原。我们使用未处理和经干扰素预处理的角质形成细胞作为刺激物，同时也作为靶细胞，以研究干扰素诱导的MHC抗原变化是否会改变角质形成细胞在同种免疫反应中的免疫原性。结果发现，携带II类MHC抗原的角质形成细胞无法诱导静息淋巴细胞增殖，但能刺激T细胞母细胞。未处理的角质形成细胞实际上对经典细胞毒性T淋巴细胞（CTL）的裂解具有抗性，但在暴露于γ干扰素后变得敏感，而在生理剂量下暴露于α2干扰素则不敏感。这些数据证明了γ干扰素的释放可能导致移植物抗宿主病等疾病发生的机制。
