Truffer Frederic, Buffi Nina, Merulla Davide, Beggah Siham, van Lintel Harald, Renaud Philippe, van der Meer Jan Roelof, Geiser Martial
Institute of Systems Engineering, University of Applied Sciences and Arts Western Switzerland Valais, Sion, Switzerland.
Laboratory of Microsystems Engineering, Ecole Polytechnique Fédérale Lausanne, EPFL-STI-LMIS, Station 17, CH-1015 Lausanne, Switzerland.
Rev Sci Instrum. 2014 Jan;85(1):015120. doi: 10.1063/1.4863333.
We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.
我们展示了一种紧凑的便携式生物传感器，用于使用大肠杆菌生物报告细胞测量水中砷（As(III)）的浓度。大肠杆菌会根据砷浓度（0至100μg/L之间）以线性依赖方式表达绿色荧光蛋白。该设备包含一个容纳琼脂糖包裹细菌的小型聚二甲基硅氧烷微流控芯片，以及一个用于自动定量荧光测量的完整光学照明/收集/检测系统。该设备能够自主采集水样、控制整个测量过程、存储数据并通过GSM网络传输数据。我们证明，该设备能够分别在100分钟和80分钟内对饮用水中10μg/L和50μg/L的砷进行高度可重复的测量。
