School of Biological Sciences, University of East Anglia, Norwich, UK.
Planta. 1968 Dec;82(4):307-16. doi: 10.1007/BF00386433.
The basipetal movement of IAA in 5-mm Zea coleoptile segments is drastically reduced under anaerobic conditions, but it remains greater than acropetal movement which is closely similar in the presence and absence of oxygen. The polarity of IAA movement has thus been confirmed in Zea coleoptile segments which have been deprived of oxygen. This net polar flux is dependent upon anaerobic metabolism since it is abolished in the presence of the metabolic inhibitiors sodium fluoride and iodoacetic acid.Acropetal movement of IAA is unaffected by the presence of sodium fluoride in air or anaerobic conditions. Uptake of IAA from a basal donor is not affected by sodium fluoride in air, but under anaerobic conditions the inhibitor decreased uptake by approximately 13%.Under anaerobic conditions both inhibitors reduce basipetal movement of IAA to the level of acropetal movement, and both decrease the total uptake of IAA from an apical donor by up to 30-45%. Under aerobic conditions sodium fluoride has no marked effect upon either the uptake of IAA from an apical donor or the basipetal movement of IAA by the segments. On the other hand, iodoacetic acid greatly decreased the uptake of IAA by the segments in air, but the same fraction of the total IAA taken up was recovered in the receiving block in the presence and absence of the inhibitor.
在缺氧条件下，5 毫米玉米胚芽鞘切段中 IAA 的向基运动大大减少，但仍大于向顶运动，而在有氧和无氧条件下，向顶运动非常相似。因此，在缺氧的玉米胚芽鞘切段中已经证实了 IAA 运动的极性。这种净向极通量依赖于无氧代谢，因为在代谢抑制剂氟化物和碘乙酸存在下，它被消除了。向顶运动的 IAA 不受空气中氟化物的影响，也不受缺氧条件的影响。在空气中，氟化物对从基部供体吸收 IAA 没有影响，但在缺氧条件下，抑制剂使吸收减少了约 13%。在缺氧条件下，两种抑制剂都将 IAA 的向基运动降低到向顶运动的水平，并将从顶端供体吸收的 IAA 总量减少 30-45%。在有氧条件下，氟化物对从顶端供体吸收 IAA 或片段中 IAA 的向基运动没有明显影响。另一方面，碘乙酸在空气中大大减少了片段对 IAA 的吸收，但在有和没有抑制剂的情况下，在接收块中回收的总 IAA 的相同分数。
