Harvard Biological Laboratories, Cambridge, Massachusetts.
Planta. 1968 Sep;83(3):223-36. doi: 10.1007/BF00385332.
As an extension of earlier cytophysiological and morphological studies on differentiating cells of Micrasterias denticulata, a fine structural investigation of glutaraldehyde-osmium tetroxide fixed material has been made. Special emphasis has been placed on the distribution of cytoplasmic microtubules and on their possible role in the processes of growth and differentiation. Four distinct systems of microtubules were found: (a) a band in the cortical protoplasm of the isthmus region which surrounds the nucleus; (b) several bands in the cortical protoplasm of the old half cells, with rod-like cross bridges between individual microtubules and between the microtubules and the plasmalemma; (c) clusters of microtubules near the posttelophase nucleus, some separated by "intertubular structures" possibly fibrils; and (d) microtubules in the internal and cortical protoplasm of differentiating half cells.
在对 Micrasterias denticulata 分化细胞的早期细胞生理学和形态学研究的基础上，我们对戊二醛-锇酸固定材料进行了精细的结构研究。特别强调了细胞质微管的分布及其在生长和分化过程中的可能作用。我们发现了四个不同的微管系统：(a) 在围绕核的峡部区域的皮质质体中存在一条带；(b) 在旧半细胞的皮质质体中有几个带，在单个微管之间以及微管与质膜之间存在杆状的横桥；(c) 在后期核附近有微管簇，一些微管通过“管间结构”（可能是原纤维）分开；(d) 在分化半细胞的内部和皮质质体中有微管。
