Sieber-Blum M, Kumar S R, Riley D A
Medical College of Wisconsin, Department of Anatomy and Cellular Biology, Milwaukee 53226.
Brain Res. 1988 Mar 1;467(1):69-83. doi: 10.1016/0165-3806(88)90068-5.
This study shows that quail neural crest cells can differentiate in vitro into sensory-like neuroblasts. The putative sensory neuroblasts were large and spherical, possessing large diameter, bipolar or pseudo-unipolar, long processes that lacked multiple varicosities characteristic of autonomic neurons. They bound HNK-1, a monoclonal antibody against a cell surface epitope expressed by early neural crest cells but not by young neural tube-derived cells. Many of the sensory-like neuroblasts had substance P (SP)-like immunoreactivity. Some exhibited histochemical carbonic anhydrase activity; carbonic anhydrase is shown in this study to stain a subpopulation of spinal sensory neurons in adult quail and embryos 9 days and older, whereas ventral root axons and neurons in sympathetic ganglia are non-reactive at all ages. Double staining indicated that unlike the multipolar neuroblasts developing in the same cultures, SP-like immunoreactive neuroblasts do not contain detectable levels of tyrosine hydroxylase or dopamine-beta-hydroxylase. Finally, the neuronal nature of the cultured sensory-like neuroblasts was further documented by double labeling for antibodies against the 68 kDa neurofilament polypeptide and substance P.
本研究表明，鹌鹑神经嵴细胞可在体外分化为感觉样神经母细胞。假定的感觉神经母细胞大且呈球形，具有大直径、双极或假单极、长突起，且缺乏自主神经元特有的多个膨体。它们结合HNK - 1，这是一种针对早期神经嵴细胞而非年轻神经管衍生细胞表达的细胞表面表位的单克隆抗体。许多感觉样神经母细胞具有P物质（SP）样免疫反应性。一些表现出组织化学碳酸酐酶活性；本研究表明碳酸酐酶可对成年鹌鹑以及9日龄及以上胚胎的脊髓感觉神经元亚群进行染色，而腹根轴突和交感神经节中的神经元在所有年龄段均无反应。双重染色表明，与在相同培养物中发育的多极神经母细胞不同，SP样免疫反应性神经母细胞不含可检测水平的酪氨酸羟化酶或多巴胺-β-羟化酶。最后，通过针对68 kDa神经丝多肽和P物质的抗体进行双重标记，进一步证明了培养的感觉样神经母细胞的神经元性质。
