黄芩苷固体分散体对D-氨基半乳糖诱导的小鼠急性肝损伤的保护作用

[Protective effect of baicalin solid dispersion on D-galactosamine induced acute hepatic injury in mice].

作者信息

Wang Chao, Nie Hao, Li Kan, Zhang Yan-Xiang, Shu Ke-Gang, Chen Xiu-Juan

机构信息

Department of Pathogenic Biology, Medical School of Yangtze University, Hubei 434023, China.

Class 10903 of Clinical Medicine, Medical School of Yangtze University, Hubei 434023, China.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Jan;34(1):71-4.

PMID:24520792

Abstract

OBJECTIVE

To study the protective effect of baicalin solid dispersion (BSD) on D-galactosamine (D-GalN) induced acute hepatic injury in mice, and to compare it with baicalin alone.

METHODS

Sixty mice were randomly divided into six groups, i.e., the normal control group, the D-GalN model group, the bifendate group (at the daily dose of 200 mg/kg), the baicalin group (at the daily dose of 50 mg/kg), the low dose BSD group (at the daily dose of 50 mg/kg), and the high dose BSD group (at the daily dose of 100 mg/kg), 10 in each group. 0.5% CMC-Na at 20 mL/kg was administered to mice in the normal group and the model group by gastrogavage, while corresponding medication was administered to mice in the other three groups by gastrogavage. Seven days after administration, acute hepatic injury model was induced by intraperitoneal injection of D-GalN. The liver index and the spleen index were calculated. The serum activities of alanine aminotransferase (ALT) and asparate aminotransferase (AST), the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver homogenate were measured. The pathological changes of the liver tissue were observed by HE staining.

RESULTS

Compared with the normal control group, widespread inflammation and necrosis was significant in the liver tissue of the D-GalN model group; the liver index, serum ALT and AST levels and hepatic MDA content obviously increased, hepatic SOD activity decreased, showing statistical difference (P < 0.05). Compared with the model group, the liver index, the serum levels of ALT and AST, and hepatic MDA decreased, hepatic SOD increased, the degree of hepatic tissue injury was significantly improved in the low dose and high dose BSD groups. Besides, better effects were obtained in the low dose BSD group than in the baicalin group with statistical difference (P < 0.05).

CONCLUSION

BSD could significantly protect D-GalN induced acute hepatic injury of mice, and its effect was superior to that of baicalin alone.

摘要

目的

研究黄芩苷固体分散体（BSD）对D-氨基半乳糖（D-GalN）诱导的小鼠急性肝损伤的保护作用，并与单独使用黄芩苷进行比较。

方法

将60只小鼠随机分为6组，即正常对照组、D-GalN模型组、联苯双酯组（每日剂量200mg/kg）、黄芩苷组（每日剂量50mg/kg）、低剂量BSD组（每日剂量50mg/kg）和高剂量BSD组（每日剂量100mg/kg），每组10只。正常组和模型组小鼠以20mL/kg的0.5%羧甲基纤维素钠（CMC-Na）灌胃给药，其他三组小鼠以相应药物灌胃给药。给药7天后，腹腔注射D-GalN诱导急性肝损伤模型。计算肝脏指数和脾脏指数。测定血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性、肝匀浆中超氧化物歧化酶（SOD）和丙二醛（MDA）含量。通过苏木精-伊红（HE）染色观察肝组织的病理变化。

结果

与正常对照组相比，D-GalN模型组肝组织出现广泛炎症和坏死；肝脏指数、血清ALT和AST水平以及肝脏MDA含量明显升高，肝脏SOD活性降低，差异具有统计学意义（P<0.05）。与模型组相比，低剂量和高剂量BSD组的肝脏指数、血清ALT和AST水平以及肝脏MDA降低，肝脏SOD升高，肝组织损伤程度明显改善。此外，低剂量BSD组的效果优于黄芩苷组，差异具有统计学意义（P<0.05）。