Jacob J
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
FEBS Lett. 1988 Apr 11;231(1):139-42. doi: 10.1016/0014-5793(88)80718-x.
The activation of functional responses in rabbit peritoneal neutrophils by gramicidin and the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine methyl ester, was studied. Gramicidin activated superoxide generation, lysosomal enzyme release and a decrease in fluorescence of chlortetracycline-loaded cells, as for the chemotactic peptide. The maximum intensities of the responses by gramicidin were lower than that by chemotactic peptide. Responses by both these peptides could be inhibited by t-butyloxycarbonyl-methionyl-leucyl-phenylalanine, a chemotactic peptide receptor antagonist. Gramicidin gave responses at low doses comparable to that of the chemotactic peptide.
研究了短杆菌肽和趋化肽N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸甲酯对兔腹膜中性粒细胞功能反应的激活作用。短杆菌肽可激活超氧化物生成、溶酶体酶释放，并使负载金霉素的细胞荧光降低，趋化肽也有同样作用。短杆菌肽引起的反应最大强度低于趋化肽。这两种肽引起的反应均可被趋化肽受体拮抗剂叔丁氧羰基-甲硫氨酰-亮氨酰-苯丙氨酸抑制。低剂量的短杆菌肽引起的反应与趋化肽相当。
