Wayne D B, Heaton M B
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610.
Dev Biol. 1988 May;127(1):220-3. doi: 10.1016/0012-1606(88)90203-5.
Neuronal retrograde transport of nerve growth factor (NGF) was examined in chick embryos at 5, 6, and 7 days of incubation. Radiolabeled NGF was injected in the target limb muscle and the retrograde transport was viewed following processing for autoradiography. Silver grains were localized in the peripheral nerve, in the ventral root, in neuronal cell bodies within the dorsal root ganglion, and in motoneurons of the lateral motor column. Comparable injections of 125I-cytochrome c resulted in the presence of label at the peripheral injection site only. The possible developmental significance of these observations is discussed.
在孵化第5、6和7天的鸡胚中检测了神经生长因子（NGF）的神经元逆行运输。将放射性标记的NGF注射到目标肢体肌肉中，经放射自显影处理后观察逆行运输情况。银颗粒定位于外周神经、腹根、背根神经节内的神经元细胞体以及外侧运动柱的运动神经元中。注射125I-细胞色素c的对照实验结果显示，标记仅出现在外周注射部位。讨论了这些观察结果可能具有的发育意义。
