Insee Jiranan, Kamolnorranath Sumate, Baicharoen Sudarat, Chumpadang Sriphapai, Sawasu Wanchai, Wajjwalku Worawidh
1 Interdisciplinary Graduate Program in Genetic Engineering. Faculty of Graduate School, Kasetsart University, Bangkok 10900, Thailand.
Zoolog Sci. 2014 Feb;31(2):95-100. doi: 10.2108/zsj.31.95.
Due to human activity and a reduction in the size and quality of wetland habitats, populations of the Eastern sarus crane (Grus antigone sharpii) have declined dramatically across their range in Southeast Asia. Conservation efforts in Thailand have focused on reintroduction of the founders harboring the highest genetic diversity. One of the most important requirements to ensure the persistence of the reintroduced populations is a balanced sex ratio. In this study we tested three simple PCR-based methods which may be used for reliable sex identification in G. a. sharpii. The first method employs two combined primer sets based on a 0.6 kb EcoRI fragment (EE0.6). The second method is based on the intronic length polymorphism of the chromo-helicase DNA binding protein (CHD). The last technique relies on PCR-RFLP technique. The sex of six known and 24 unknown cranes were successfully identified by all three methods. These PCR-based sex identification methods are also useful for captive breeding management of G. a. sharpii.
由于人类活动以及湿地栖息地面积和质量的减少，东南亚地区东部食猿雕（Grus antigone sharpii）的种群数量在其分布范围内急剧下降。泰国的保护工作重点是重新引入具有最高遗传多样性的奠基者。确保重新引入种群持续存在的最重要要求之一是性别比例平衡。在本研究中，我们测试了三种基于简单PCR的方法，这些方法可用于可靠地鉴定食猿雕的性别。第一种方法使用基于0.6 kb EcoRI片段（EE0.6）的两个组合引物组。第二种方法基于染色体解旋酶DNA结合蛋白（CHD）内含子长度多态性。最后一种技术依赖于PCR-RFLP技术。所有三种方法都成功鉴定了6只已知和24只未知食猿雕的性别。这些基于PCR的性别鉴定方法也有助于食猿雕的圈养繁殖管理。
