单个初级听神经元的钴标记：辣根过氧化物酶的替代方法。
Cobalt labelling of single primary auditory neurones: an alternative to HRP.
作者信息
Köppl C, Gleich O
机构信息
Institut für Zoologie der Technischen Universität München, Garching, F.R.G.
出版信息
Hear Res. 1988 Feb-Mar;32(2-3):111-6. doi: 10.1016/0378-5955(88)90082-2.
We have labelled single, primary auditory neurones in three reptile and one bird species. After functional characterization of the neurones, hexamminecobaltic chloride was iontophoretically injected through the recording micropipette. Precipitation of cobalt sulfide followed by silver intensification of the cochlear duct as a whole-mount preparation revealed stained neurones in over 90% of cases. This method has several advantages over labelling with HRP.
我们已标记了三种爬行动物和一种鸟类的单个初级听觉神经元。在对神经元进行功能特性鉴定后，通过记录微电极以离子电泳方式注入六氨合氯化钴。硫化钴沉淀后，对整个耳蜗管进行银增强染色，在超过90%的案例中显示出被染色的神经元。与用辣根过氧化物酶（HRP）标记相比，这种方法有几个优点。
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