囊性纤维化患者血清中抗铜绿假单胞菌抗体作为诊断工具的系统评价
Serum antibodies to Pseudomonas aeruginosa in cystic fibrosis as a diagnostic tool: a systematic review.
作者信息
Mauch Renan Marrichi, Levy Carlos Emilio
机构信息
Faculty of Medical Sciences, State University of Campinas, Brazil.
Departament of Clinic Pathology, Hospital de Clínicas, Faculty of Medical Sciences, State University of Campinas, Brazil.
出版信息
J Cyst Fibros. 2014 Sep;13(5):499-507. doi: 10.1016/j.jcf.2014.01.005. Epub 2014 Feb 9.
BACKGROUND
A systematic literature review of the last 40 years on the research of serum antibodies to Pseudomonas aeruginosa in cystic fibrosis and its utility as a diagnostic tool.
METHODS
Research papers in English, Portuguese, and Spanish were accessed through electronic databases (PubMed, Medline, LILACS, and SciELO).
RESULTS
26 studies were assessed. ELISA technique was the most commonly used technique to detect serum P. aeruginosa antibodies. The most consistent results were those in which the response against the antigen St-Ag:1-17 was evaluated. The accuracy levels of the ELISA technique remain controversial, but most studies showed a good correlation between antibody titers and microbiological culture.
CONCLUSIONS
The detection of serum antibodies to P. aeruginosa shows capacity for early detection of this pathogen and potential utility and viability of incorporation in the diagnostic routine of patients with cystic fibrosis.
背景
对过去40年关于囊性纤维化患者血清抗铜绿假单胞菌抗体研究及其作为诊断工具的效用进行系统文献综述。
方法
通过电子数据库（PubMed、Medline、LILACS和SciELO）检索英文、葡萄牙文和西班牙文的研究论文。
结果
评估了26项研究。ELISA技术是检测血清铜绿假单胞菌抗体最常用的技术。针对抗原St-Ag:1-17的反应评估结果最为一致。ELISA技术的准确性水平仍存在争议，但大多数研究表明抗体滴度与微生物培养之间具有良好的相关性。
结论
检测血清抗铜绿假单胞菌抗体显示出早期检测该病原体的能力，以及将其纳入囊性纤维化患者诊断常规的潜在效用和可行性。
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