Antibody-guided identification of protein antigens in cystic fibrosis.

Persistent bacterial airway infection is a hallmark feature of cystic fibrosis (CF). spp. are gram-negative rods that can cause persistent airway infection in people with CF (pwCF), but the knowledge of host immune responses to these bacteria is limited. The aim of this study was to investigate if patients develop antibodies against , the most common species, and to identify the bacterial antigens that induce specific IgG responses. Seven serum samples from pwCF with infection were screened for antibodies against bacteria in an ELISA coated with , or . Sera from pwCF with or without infection ( = 22 and 20, respectively) and healthy donors ( = 4) were included for comparison. Serum with high titers to was selected for affinity purification of bacterial antigens using serum IgGs bound to protein G beads. The resulting IgG-antigen complexes were then analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Selected antigens of interest were produced in recombinant form and used in an ELISA to confirm the results. Four of the seven patients with infection had serum antibodies against . Using patient serum-IgG for affinity purification of proteins, we identified eight antigens. Three of these, which were not targeted by anti-. antibodies, were expressed recombinantly for further validation: dihydrolipoyl dehydrogenase (DLD), type I secretion C-terminal target domain-containing protein, and domain of uncharacterized function 336 (DUF336). While specific IgG against all three recombinant antigens was confirmed in the patient serum with high titers against , DLD and DUF336 showed the least binding to serum IgG from pwCF without spp. infection. Using serum IgG affinity purification in combination with LC-MS/MS and confirming the results using ELISA against recombinant proteins, we have identified bacterial antigens from .IMPORTANCE species are opportunistic pathogens that can cause airway infections in people with cystic fibrosis. In this patient population, persistent infection is associated with low lung function, but the knowledge about bacterial interactions with the host is currently limited. In this study, we identify protein antigens that induce specific antibody responses in the host. The identified antigens may potentially be useful in serological assays, serving as a complement to culturing methods for the diagnosis and surveillance of infection.