Biological Institute, Faculty of Science, Nagoya University, Nagoya, Japan.
Planta. 1967 Jun;77(2):127-34. doi: 10.1007/BF00387449.
Prolonged irradiation with red light and addition of sucrose to the basal medium induced two-dimensional growth in gametophytes of Pteris vittata. The results suggest that two-dimensional growth is controlled by the following three factors: 1. a photoreactive system which depends on blue light; 2. a photoreactive system which can utilize red light; and 3. the photosynthetic system which the level of carbohydrate. Media containing 2.4-dichlorophenoxyacetic acid and sucrose, and complex media containing peptone caused formation of tumor-like calli.
红光长时间照射和在基础培养基中添加蔗糖诱导凤尾蕨配子体的二维生长。结果表明,二维生长受以下三个因素控制:1. 依赖蓝光的光反应系统;2. 可以利用红光的光反应系统;3. 碳水化合物水平的光合系统。含有 2,4-二氯苯氧乙酸和蔗糖的培养基和含有蛋白胨的复杂培养基导致肿瘤样愈伤组织的形成。