Yan Wei, Zhou Huitong, Luo Yuzhu, Hu Jiang, Hickford Jon G H
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
PLoS One. 2014 Feb 11;9(2):e88691. doi: 10.1371/journal.pone.0088691. eCollection 2014.
The fatty acid binding protein 4 (FABP4) plays an important role in the regulation of lipid metabolism in mammals. In this study, two regions of ovine FABP4 spanning exon 2-intron 2 and exon 3-intron 3 were investigated in four hundred and twenty lambs derived from seven sires that were previously typed as having heterozygous genotypes in both these regions of the gene. These regions have been shown to be variable, with three SNPs plus one indel and four SNPs respectively constituting five and four allele variants in the two regions. Across these regions, fourteen haplotypes have been identified. The lambs were typed using a Polymerase Chain Reaction Single-Stranded Conformational Polymorphism (PCR-SSCP) method to identify the haplotypes inherited from the sires. Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4. A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.
脂肪酸结合蛋白4（FABP4）在哺乳动物脂质代谢调节中发挥重要作用。在本研究中，对来自7只公羊的420只羔羊的绵羊FABP4跨越外显子2-内含子2和外显子3-内含子3的两个区域进行了研究，这7只公羊先前在该基因的这两个区域被鉴定为杂合基因型。这些区域已被证明是可变的，在这两个区域中分别有3个单核苷酸多态性（SNP）加上1个插入缺失和4个SNP，分别构成5个和4个等位基因变体。在这些区域中，已鉴定出14种单倍型。使用聚合酶链反应单链构象多态性（PCR-SSCP）方法对羔羊进行分型，以鉴定从公羊遗传而来的单倍型。在7只公羊中的6只公羊的后代中鉴定出3至4种父本来源的单倍型，这表明减数分裂重组发生在绵羊FABP4内。在分析的基因的两个区域中检测到一些与重组“热点”相关的序列基序，这些可能促进重组。
