Animal Genome & Bioinformatics Division, National Institute of Animal Science, RDA, Suwon 441-701, Korea.
Anim Genet. 2010 Aug;41(4):442-4. doi: 10.1111/j.1365-2052.2010.02024.x. Epub 2010 Mar 15.
The objective of this study was to investigate an association between polymorphisms in the FABP4 gene and phenotypic variation for marbling and carcass weight (CWT) in a population of Hanwoo steers. We re-sequenced 4.3 kb of the FABP4 gene region in 24 Hanwoo bulls and identified 16 SNPs and 1 microsatellite polymorphism. Of these 16 SNPs, three SNPs [g.2774G>C (intron I), g.3473A>T (intron II) and g.3631G>A (exon III, creating a p.Met >Val amino acid substitution)] were genotyped in 583 steers to assess their association with carcass traits. The g.3473A allele showed a significant increasing effect on CWT (P = 0.01) and the g.3631G allele was associated with higher marbling score (P = 0.006). One haplotype of these three SNPs (CAG) was significantly associated with CWT (P = 0.02) and marbling score (P = 0.05) and could potentially be of value for marker assisted selection in Hanwoo cattle. The CAG haplotype effect for CWT was larger (11.14 +/- 5.03 kg) than the largest single locus effect of g.3473A>T (5.01 +/- 2.2 kg).
本研究旨在探讨 FABP4 基因多态性与韩牛公牛大理石花纹和胴体体重（CWT）表型变异之间的关系。我们对 24 头韩牛公牛的 FABP4 基因区域进行了 4.3 kb 的重测序，鉴定出 16 个 SNPs 和 1 个微卫星多态性。在这 16 个 SNP 中，有 3 个 SNP [g.2774G>C（内含子 I）、g.3473A>T（内含子 II）和 g.3631G>A（外显子 III，导致 p.Met > Val 氨基酸取代)]在 583 头公牛中进行了基因分型，以评估它们与胴体性状的关系。g.3473A 等位基因对 CWT 有显著的正向影响（P = 0.01），g.3631G 等位基因与较高的大理石花纹评分相关（P = 0.006）。这三个 SNP 的一个单倍型（CAG）与 CWT（P = 0.02）和大理石花纹评分（P = 0.05）显著相关，可能对韩牛的标记辅助选择有价值。CAG 单倍型对 CWT 的影响较大（11.14 +/- 5.03 kg），大于 g.3473A>T 的最大单一位点效应（5.01 +/- 2.2 kg）。
