Zandén Carl, Hellström Erkenstam Nina, Padel Thomas, Wittgenstein Julia, Liu Johan, Kuhn H Georg
Bionano Systems Laboratory, Department of Microtechnology and Nanoscience (MC2), Chalmers University of Technology, Göteborg, Sweden.
Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
Nanomedicine. 2014 Jul;10(5):949-58. doi: 10.1016/j.nano.2014.01.010. Epub 2014 Feb 10.
The topographical effects from functional materials on stem cell behavior are currently of interest in tissue engineering and regenerative medicine. Here we investigate the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell (hESC) and rat postnatal neural stem cell (NSC) responses. The plasma gases were found to induce three combinations of fiber surface functionalities and roughness textures. On randomly oriented fibers, plasma treatments lead to substantially increased hESC attachment and proliferation as compared to native fibers. Argon plasma was found to induce the most optimal combination of surface functionality and roughness for cell expansion. Contact guided migration of cells and alignment of cell processes were observed on aligned fibers. Neuronal differentiation around 5% was found for all samples and was not significantly affected by the induced variations of surface functional group distribution or individual fiber topography.
In this study the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell and rat postnatal neural stem cell (NSC) responses is studied with the goal of clarifying the potential effects of functional materials on stem cell behavior, a topic of substantial interest in tissue engineering and regenerative medicine.
功能材料对干细胞行为的地形学效应目前在组织工程和再生医学领域备受关注。在此，我们研究了电纺聚氨酯纤维经氩气、氧气和氢气等离子体表面改性后对人胚胎干细胞（hESC）和大鼠出生后神经干细胞（NSC）反应的影响。发现等离子体气体可诱导纤维表面功能和粗糙度纹理的三种组合。在随机取向的纤维上，与天然纤维相比，等离子体处理导致hESC的附着和增殖显著增加。发现氩等离子体可诱导出细胞扩增的最理想表面功能和粗糙度组合。在排列的纤维上观察到细胞的接触引导迁移和细胞突起的排列。所有样品的神经元分化率约为5%，且未受到表面官能团分布或单个纤维地形诱导变化的显著影响。
在本研究中，研究了电纺聚氨酯纤维经氩气、氧气和氢气等离子体表面改性后对人胚胎干细胞和大鼠出生后神经干细胞（NSC）反应的影响，目的是阐明功能材料对干细胞行为的潜在影响，这是组织工程和再生医学领域一个备受关注的话题。
