Department of Physics, University of Exeter, Stocker Road, Exeter EX4 4QL, UK.
Department of Computer Science, University of Exeter, North Park Road, Exeter EX4 4QF, UK.
Acta Biomater. 2014 Jun;10(6):2574-81. doi: 10.1016/j.actbio.2014.02.008. Epub 2014 Feb 11.
Articular cartilage (AC) is a highly anisotropic biomaterial, and its complex mechanical properties have been a topic of intense investigation for over 60 years. Recent advances in the field of nonlinear optics allow the individual constituents of AC to be imaged in living tissue without the need for exogenous contrast agents. Combining mechanical testing with nonlinear microscopy provides a wealth of information about microscopic responses to load. This work investigates the inhomogeneous distribution of strain in loaded AC by tracking the movement and morphological changes of individual chondrocytes using point pattern matching and Bayesian modeling. This information can be used to inform models of mechanotransduction and pathogenesis, and is readily extendable to various other connective tissues.
关节软骨（AC）是一种各向异性的生物材料，其复杂的力学性质已经成为 60 多年来的研究热点。非线性光学领域的最新进展使得人们无需外源性对比剂即可对活体组织中的 AC 的各个成分进行成像。将力学测试与非线性显微镜相结合，可以提供大量关于微观组织对负载响应的信息。本工作通过使用点模式匹配和贝叶斯建模来跟踪单个软骨细胞的运动和形态变化，研究了受载 AC 中的应变不均匀分布。该信息可用于为力学转导和发病机制的模型提供信息，并且易于扩展到各种其他结缔组织。
