一种用于脂肪酶合成活性测定的灵敏比色高通量筛选方法。
A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay.
作者信息
Zheng Jianyong, Fu Xianfeng, Ying Xiangxian, Zhang Yinjun, Wang Zhao
机构信息
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
出版信息
Anal Biochem. 2014 May 1;452:13-5. doi: 10.1016/j.ab.2014.02.001. Epub 2014 Feb 10.
A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.
本文描述了一种用于测定脂肪酶合成活性的灵敏且实用的高通量筛选方法。选择脂肪酶催化乙酸乙烯酯与正丁醇在正己烷中的酯交换反应作为模型反应。使用3-甲基-2-苯并噻唑啉酮（MBTH）衍生化的比色法测定释放的乙醛。与其他方法相比，该方法的主要优点包括高灵敏度、检测简单、试剂成本低以及对仪器要求低。
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