Haaf T, Ott G, Schmid M
Institut für Humangenetik der Universität, Würzburg, Federal Republic of Germany.
Hum Genet. 1988 May;79(1):18-23. doi: 10.1007/BF00291703.
The cytidine analogue 5-azadeoxycytidine (5-azadC) induces a very distinct inhibition of condensation in the genetically inactive, late-replicating X chromosome (XL) when applied to human lymphocyte cultures. One of the two X chromosomes in cytogenetically normal female cells becomes dramatically longer than its homologous partner. The highest rate of metaphases with an undercondensed XL chromosome is achieved when 5-aza-dC is added at a final concentration of 10(-5) M 2 h before cell harvesting. The interactions between 5-aza-dC and chromosomal DNA as well as the factors involved in X chromosome inactivation are discussed.
胞苷类似物5-氮杂脱氧胞苷（5-azadC）应用于人类淋巴细胞培养物时，会对遗传上无活性、复制较晚的X染色体（XL）的凝聚产生非常明显的抑制作用。细胞遗传学正常的女性细胞中的两条X染色体之一会比其同源配对染色体长得多。在细胞收获前2小时添加终浓度为10^(-5) M的5-氮杂-dC时，XL染色体凝聚不足的中期细胞比例最高。本文讨论了5-氮杂-dC与染色体DNA之间的相互作用以及参与X染色体失活的因素。
