Wang Kai, Liu Chao, Di Chan-Juan, Ma Cong, Han Chun-Guang, Yuan Mei-Ru, Li Peng-Fei, Li Lu, Liu Yong-Xue
School of Graduate Studies, Anhui Medical University, Hefei, China E-mail :
Asian Pac J Cancer Prev. 2014;15(1):291-7. doi: 10.7314/apjcp.2014.15.1.291.
The radioprotective effects of a single administration of kojic acid (KA) against ionizing radiation were evaluated via assessment of 30-day survival and alterations of peripheral blood parameters of adult C57BL/6 male mice. The 30-day survival rate of mice pretreated with KA (75 or 300 mg/kg body weight, KA75 or KA300) subcutaneously 27 h prior to a lethal dose (8 Gy, 153.52 cGy/min) of gamma irradiation was higher than that of mice irradiated alone (40% or 60% vs 0%). It was observed that the white blood cell (WBC) count/the red blood cell (RBC) count, haemoglobin content, haematocrit and platelet count of mice with or without KA pretreatment as exposed to a sub-lethal dose (4 Gy, 148.14 cGy/min) of gamma irradiation decreased maximally at day 4/day 8 post-irradiation. Although the initial WBC values were low in KA300 or WR-2721 (amifostine) groups, they significantly recovered to normal at day 19, whereas in the control group they did not. The results from the cytotoxicity and cell viability assays demonstrated that KA could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity. In summary, KA provides marked radioprotective effects both in vivo and in vitro.
通过评估成年C57BL/6雄性小鼠30天生存率及外周血参数变化，对单次给予曲酸（KA）的电离辐射防护作用进行了评价。在致死剂量（8 Gy，153.52 cGy/min）γ射线照射前27小时，皮下注射KA（75或300 mg/kg体重，KA75或KA300）预处理的小鼠30天生存率高于单纯照射小鼠（40%或60%对0%）。观察到，接受或未接受KA预处理的小鼠在接受亚致死剂量（4 Gy，148.14 cGy/min）γ射线照射后，白细胞（WBC）计数/红细胞（RBC）计数、血红蛋白含量、血细胞比容和血小板计数在照射后第4天/第8天降至最低。虽然KA300或WR-2721（氨磷汀）组初始WBC值较低，但在第19天显著恢复正常，而对照组则未恢复。细胞毒性和细胞活力分析结果表明，KA能高效保护中国仓鼠卵巢（CHO）细胞免受电离辐射，且毒性较低。总之，KA在体内和体外均具有显著的辐射防护作用。
