脑膜瘤中O6-甲基鸟嘌呤甲基转移酶基因启动子的甲基化——I、II和III级肿瘤之间的比较
Methylation of O6-methyl guanine methyltransferase gene promoter in meningiomas--comparison between tumor grades I, II, and III.
作者信息
Larijani Leila, Madjd Zahra, Samadikuchaksaraei Ali, Younespour Shima, Zham Hanieh, Rakhshan Azadeh, Mohammadi Foruzan, Rahbari Ali, Moradi Afshin
机构信息
Department of Biology, School of Basic Sciences, Science and Research Branch Islamic Azad University (IAU), Tehran, Iran E-mail :
出版信息
Asian Pac J Cancer Prev. 2014;15(1):33-8. doi: 10.7314/apjcp.2014.15.1.33.
BACKGROUND
Meningiomas are the second most common primary intracranial tumors after gliomas. Epigenetic biomarkers such as DNA methylation, which is found in many tumors and is thus important in tumorigenesis can help diagnose meningiomas and predict response to adjuvant chemotherapy. We investigated aberrant O6- methyl guanine methyltransferase (MGMT) methylation in meningiomas.
MATERIALS AND METHODS
Sixty-one patients were classified according to the WHO grading, and MGMT promoter methylation status was examined via the methylation-Specific PCR(MSP) method.
RESULTS
MGMT promoter methylation was found in 22.2% of grade I, 35% of grade I with atypical features, 36% of grade II, and 42.9% of grade III tumors.
CONCLUSIONS
There was an increase, albeit not statistically significant, in MGMT methylation with a rise in the tumor grade. Higher methylation levels were also observed in the male gender.
背景
脑膜瘤是继胶质瘤之后第二常见的原发性颅内肿瘤。表观遗传生物标志物如DNA甲基化在许多肿瘤中都有发现，因此在肿瘤发生过程中很重要，可帮助诊断脑膜瘤并预测辅助化疗的反应。我们研究了脑膜瘤中异常的O6-甲基鸟嘌呤甲基转移酶（MGMT）甲基化情况。
材料与方法
61例患者根据世界卫生组织（WHO）分级进行分类，并通过甲基化特异性PCR（MSP）方法检测MGMT启动子甲基化状态。
结果
在I级肿瘤中，22.2%发现MGMT启动子甲基化；在具有非典型特征的I级肿瘤中，35%发现；在II级肿瘤中，36%发现；在III级肿瘤中，42.9%发现。
结论
MGMT甲基化随肿瘤分级升高而增加，尽管无统计学意义。在男性中也观察到较高的甲基化水平。
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