Gopal Priya Kalyan, Paul Mausumi, Paul Santanu
Laboratory of Experimental Immunology, Department of Microbiology and Botany, Gurudas College, Kolkata, India E-mail :
Asian Pac J Cancer Prev. 2014;15(1):93-100. doi: 10.7314/apjcp.2014.15.1.93.
Curcumin has has been reported to exert anti-inflammatory, anti-oxidation and anti-angiogenic activity in various types of cancer. It has also been shown to induce apoptosis in leukemia cells. We aimed to unravel the role of the redox pathway in Curcumin mediated apoptosis with a panel of human leukemic cells.
In this study in vitro cytotoxicity of Curcumin was measured by MTT assay and apoptotic effects were assessed by annexin V/PI, DAPI staining, cell cycle analysis, measurement of caspase activity and PARP cleavage. Effects of Curcumin on intracellular redox balance were assessed using fluorescent probes like H2DCFDA, JC1 and an ApoGSH Glutathione Detection Kit respectively.
Curcumin showed differential anti-proliferative and apoptotic effects on different human leukemic cell lines in contrast to minimal effects on normal cells. Curcumin induced apoptosis was associated with the generation of intracellular ROS, loss of mitochondrial membrane potential, intracellular GSH depletion, caspase activation.
As Curcumin induces programmed cell death specifically in leukemic cells it holds a great promise as a future therapeutic agent in the treatment of leukemia.
据报道，姜黄素在各类癌症中具有抗炎、抗氧化和抗血管生成活性。它还被证明可诱导白血病细胞凋亡。我们旨在通过一组人类白血病细胞来阐明氧化还原途径在姜黄素介导的凋亡中的作用。
在本研究中，通过MTT法测定姜黄素的体外细胞毒性，并通过膜联蛋白V/碘化丙啶、4',6-二脒基-2-苯基吲哚染色、细胞周期分析、半胱天冬酶活性测定和聚（ADP-核糖）聚合酶裂解来评估凋亡效应。分别使用2',7'-二氯二氢荧光素二乙酸酯、JC-1等荧光探针和ApoGSH谷胱甘肽检测试剂盒评估姜黄素对细胞内氧化还原平衡的影响。
与对正常细胞的最小影响相比，姜黄素对不同人类白血病细胞系表现出不同的抗增殖和凋亡作用。姜黄素诱导的凋亡与细胞内活性氧的产生、线粒体膜电位的丧失、细胞内谷胱甘肽的消耗、半胱天冬酶的激活有关。
由于姜黄素特异性地诱导白血病细胞发生程序性细胞死亡，它作为未来治疗白血病的药物具有很大的前景。
