Gregersen J P, Wege H, Preiss L, Jentsch K D
Research Laboratories of Behringwerke AG, Marburg, F.R.G.
J Virol Methods. 1988 Feb;19(2):161-8. doi: 10.1016/0166-0934(88)90159-0.
A micromethod for the detection of human immunodeficiency virus (HIV) and other retroviruses in cell culture supernatants is described which applies a DEAE ion exchanger for recovery of polynucleotides synthesized in vitro by the retroviral reverse transcriptase. Cell culture, sample preparation, and test performance including the washing step are adapted to microtitre plates. Compared to the standard method this technique produced less non-specific reactions, resulting in a more than 3-fold higher sensitivity, a higher reproducibility due to lower intrarun variations and allowed an increase in the daily sample accomplishment per person 3- to 4-fold at lower costs per sample.
本文描述了一种用于检测细胞培养上清液中人类免疫缺陷病毒（HIV）和其他逆转录病毒的微量方法，该方法应用DEAE离子交换剂来回收由逆转录病毒逆转录酶在体外合成的多核苷酸。细胞培养、样品制备以及包括洗涤步骤在内的检测操作均适用于微量滴定板。与标准方法相比，该技术产生的非特异性反应更少，灵敏度提高了3倍以上，由于批内变异较低，重现性更高，并且每人每天的样品处理量增加了3至4倍，同时每个样品的成本更低。
