Akhtar Reyhan, Ali Mohd, Mahmood Safrunnisa, Sanyal Sankar Nath
Department of Pharmacology Postgraduate Institute of Medical Education and Research. Chandigarh..
Faculty of Pharmacy. Jamia Hamdard. New Delhi..
Nutr Hosp. 2014 Feb 1;29(2):388-92. doi: 10.3305/nh.2014.29.2.7080.
Silibinin a flavonoid from milk thistle (Silybum marianum) exhibit a variety of pharmacological actions, including anti-proliferative and apoptotic activities against various types of cancers in intact animals and cancer cell lines. In the present study, the effect of silibinin on human colon cancer HT-29 cells was studied.
Incubations of cells with different silibinin concentrations (0.783-1,600 ug/ml) for 24, 48 or 72 h showed a progressive decline in cell viability.
Loss of cell viability was time dependent and optimum inhibition of cell growth (78%) was observed at 72 h. Under inverted microscope, the dead cells were seen as cell aggregates. IC50 (silibinin concentration killing 50% cells) values were 180, 110 and 40 ug/ml at 24, 48 and 72 h respectively.
These findings re-enforce the anticancer potential of silibinin, as reported earlier for various other cancer cell lines (Ramasamy and Agarwal (2008), Cancer Letters, 269: 352-62).
水飞蓟宾是一种来自水飞蓟（Silybum marianum）的黄酮类化合物，在完整动物和癌细胞系中表现出多种药理作用，包括对各种类型癌症的抗增殖和凋亡活性。在本研究中，研究了水飞蓟宾对人结肠癌HT-29细胞的作用。
用不同浓度（0.783 - 1600微克/毫升）的水飞蓟宾处理细胞24、48或72小时，细胞活力呈进行性下降。
细胞活力丧失呈时间依赖性，在72小时时观察到对细胞生长的最佳抑制（78%）。在倒置显微镜下，死细胞表现为细胞聚集体。在24、48和72小时时，半数抑制浓度（水飞蓟宾杀死50%细胞的浓度）值分别为180、110和40微克/毫升。
这些发现进一步证实了水飞蓟宾的抗癌潜力，正如之前对各种其他癌细胞系所报道的那样（Ramasamy和Agarwal（2008年），《癌症通讯》，269：352 - 62）。
