Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Department of Intelligent Machines and System Engineering, Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, Japan; Department of Mechanical Systems Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Japan.
Carbohydr Polym. 2014 Mar 15;103:538-49. doi: 10.1016/j.carbpol.2013.12.083. Epub 2014 Jan 7.
Biological activities of salmon nasal cartilage proteoglycan fractions are known, however, structural information is lacking. Recently, the major proteoglycan of this cartilage was identified as aggrecan. In this study, global molecular images and glycosaminoglycan structure of salmon nasal cartilage aggrecan purified from 4M guanidine hydrochloride extract were analyzed using HPLCs and atomic force microscopy with bovine tracheal cartilage aggrecan as a control. The estimated numbers of sulfates per disaccharide unit of chondroitin sulfate chains of salmon and bovine aggrecans were similar (approximately 0.85). However, the disaccharide composition showed a higher proportion of chondroitin 6-sulfate units in salmon aggrecan, 60%, compared to 40% in bovine. Gel filtration HPLC and monosaccharide analysis showed the salmon aggrecan had a lower number (approximately one-third), but 1.5-3.3 times longer chondroitin sulfate chains than the bovine aggrecan. Atomic force microscopic molecular images of aggrecan supported the images predicted by biochemical analyses.
鲑鱼鼻甲骨蛋白聚糖组分的生物学活性是已知的，然而，其结构信息却缺乏。最近，这种软骨的主要蛋白聚糖被鉴定为聚集蛋白聚糖。在这项研究中，使用高效液相色谱法和原子力显微镜分析了从 4M 盐酸胍提取物中纯化的鲑鱼鼻甲骨聚集蛋白聚糖的整体分子图像和糖胺聚糖结构，并以牛气管软骨聚集蛋白聚糖作为对照。鲑鱼和牛聚集蛋白聚糖的软骨素硫酸盐链每二糖单位的硫酸盐数量估计相似（约 0.85）。然而，二糖组成显示鲑鱼聚集蛋白聚糖中软骨素 6-硫酸盐单位的比例较高，为 60%，而牛聚集蛋白聚糖中为 40%。凝胶过滤高效液相色谱和单糖分析表明，鲑鱼聚集蛋白聚糖的硫酸软骨素链数量较少（约三分之一），但长度是牛聚集蛋白聚糖的 1.5-3.3 倍。聚集蛋白聚糖的原子力显微镜分子图像支持了生化分析预测的图像。
