Martins Patrícia Rocha, Nascimento Rodolfo Duarte, de Souza Lisboa André, Martinelli Patrícia Massara, d'Ávila Reis Débora
Department of Morphology, ICB, Universidade Federal de Minas Gerais, 31.270-901, Brazil.
Department of Morphology, ICB, Universidade Federal de Minas Gerais, 31.270-901, Brazil.
Hum Immunol. 2014 Apr;75(4):302-5. doi: 10.1016/j.humimm.2014.02.003. Epub 2014 Feb 12.
Tryptase and chymase are mast cell (MC)-specific proteases, which influence in the activation of inflammatory cells. In this study, we quantified tryptase- or chymase-expressing MCs in the oesophaguses of Chagas patients, and searched for a correlation between those data with area of nerve fibres that expressed either PGP9.5 (pan-marker) or vasoactive intestinal polypeptide (VIP), which is a neuromediator that has anti-inflammatory activity. Samples from the oesophaguses of 14 individuals Trypanosoma cruzi-infected and from six uninfected individuals were analysed by immunohistochemistry. It was demonstrated that the number of tryptase-IR MCs in infected individuals increased when compared with controls, regardless of whether the individuals had megaoesophagus, whereas the number of chymase-IR MCs increased only in infected individuals without megaoesophagus. Negative correlations were observed between tryptase-IR MCs and the density of nerve fibres that expressed VIP or PGP 9.5-IR. The participation of chymase and tryptase in this type of immunopathology is discussed.
类胰蛋白酶和糜蛋白酶是肥大细胞（MC）特异性蛋白酶，它们影响炎症细胞的激活。在本研究中，我们对恰加斯病患者食管中表达类胰蛋白酶或糜蛋白酶的MC进行了定量，并寻找这些数据与表达PGP9.5（泛标记物）或血管活性肠肽（VIP，一种具有抗炎活性的神经介质）的神经纤维面积之间的相关性。通过免疫组织化学分析了14例克氏锥虫感染个体和6例未感染个体的食管样本。结果表明，与对照组相比，感染个体中类胰蛋白酶免疫反应性MC的数量增加，无论个体是否患有巨食管，而糜蛋白酶免疫反应性MC的数量仅在没有巨食管的感染个体中增加。在表达VIP或PGP 9.5免疫反应性的神经纤维密度与类胰蛋白酶免疫反应性MC之间观察到负相关。本文讨论了糜蛋白酶和类胰蛋白酶在这种免疫病理学类型中的作用。
