利尿剂对蛙离体感觉神经元中γ-氨基丁酸门控氯电流的影响。
Effects of diuretics on GABA-gated chloride current in frog isolated sensory neurones.
作者信息
Inomata N, Ishihara T, Akaike N
机构信息
Laboratory of Experimental Pharmacology, Suntory Institute for Biomedical Research, Osaka, Japan.
出版信息
Br J Pharmacol. 1988 Mar;93(3):679-83. doi: 10.1111/j.1476-5381.1988.tb10326.x.
Abstract
- Effects of three diuretics (furosemide, amiloride and alpha-human atrial natriuretic polypeptide (alpha-hANP] on GABA-activated chloride current (ICl) were investigated in frog isolated sensory neurones, following suppression of Na+, K+ and Ca2+ currents, by use of a 'concentration-clamp' technique. 2. Furosemide inhibited the GABA-activated ICl in a non-competitive manner and facilitated the inactivation phase, while amiloride inhibited the GABA response in a competitive manner, both inhibitions being concentration-dependent. Alpha-hANP had no effects on the GABA-induced ICl. 3. The reversal potential of GABA-activated ICl (EGABA) was not shifted in the presence of amiloride or furosemide. 4. The results suggest that amiloride may act at the GABA binding site while furosemide may act on the GABA-gated chloride channel.
摘要
- 采用“浓度钳”技术，在抑制蛙离体感觉神经元的Na⁺、K⁺和Ca²⁺电流后，研究了三种利尿剂（呋塞米、阿米洛利和α-人心房利钠多肽（α-hANP））对GABA激活的氯离子电流（ICl）的影响。2. 呋塞米以非竞争性方式抑制GABA激活的ICl，并促进失活期，而阿米洛利以竞争性方式抑制GABA反应，两种抑制均呈浓度依赖性。α-hANP对GABA诱导的ICl无影响。3. 在存在阿米洛利或呋塞米的情况下，GABA激活的ICl的反转电位（EGABA）未发生偏移。4. 结果表明，阿米洛利可能作用于GABA结合位点，而呋塞米可能作用于GABA门控氯离子通道。
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