Deutsch U, Dressler G R, Gruss P
Max Planck Institute for Biophysical Chemistry, Göttingen, Federal Republic of Germany.
Cell. 1988 May 20;53(4):617-25. doi: 10.1016/0092-8674(88)90577-6.
The mouse genome contains at least three copies of sequences homologous to the "paired box", a conserved domain in several Drosophila segmentation genes of the pair-rule and segment polarity classes. Overlapping phages were isolated from two different genomic libraries using the Drosophila gooseberry distal paired box as a probe. The hybridizing sequences are highly homologous to the conserved Drosophila paired box sequences. A single 3.1 kb Pax 1 (paired box gene) transcript was detected during embryonic development, whereas no transcripts were detected in adult tissues. Detailed in situ hybridization analyses with frozen embryonic sections demonstrated Pax 1 transcripts in the perichordal zone of the developing vertebral column. The expression pattern suggests a role for this gene in the formation of segmented structures of the mouse embryo.
小鼠基因组包含至少三个与“配对盒”同源的序列拷贝，“配对盒”是成对规则和体节极性类别的几个果蝇体节基因中的一个保守结构域。使用果蝇醋栗远端配对盒作为探针，从两个不同的基因组文库中分离出重叠噬菌体。杂交序列与保守的果蝇配对盒序列高度同源。在胚胎发育过程中检测到一个单一的3.1 kb Pax 1（配对盒基因）转录本，而在成年组织中未检测到转录本。对冷冻胚胎切片进行的详细原位杂交分析表明，在发育中的脊柱的脊索区存在Pax 1转录本。这种表达模式表明该基因在小鼠胚胎节段性结构的形成中起作用。
