Endocrinologie de la Reproduction, Centre Hospitalier Universitaire de Québec, Hôpital Saint-François d'Assise, Faculté de Médecine, Université Laval, Montreal, Quebec, Canada.
Endocrinologie de la Reproduction, Centre Hospitalier Universitaire de Québec, Hôpital Saint-François d'Assise, Faculté de Médecine, Université Laval, Montreal, Quebec, Canada; Département d'Obstétrique, Gynécologie et Reproduction, Faculté de Médecine, Université Laval, Montreal, Quebec, Canada.
Fertil Steril. 2014 Apr;101(4):1183-93. doi: 10.1016/j.fertnstert.2014.01.011. Epub 2014 Feb 15.
To investigate the expression kinetics of interleukin-1 receptors (IL-1R), receptor antagonist (IL-1RN), and monocyte chemotactic protein 1 (MCP-1) throughout early gestation in mice.
Assessment of IL-1R, IL-1RN, and MCP-1 throughout early pregnancy.
Reproduction laboratory.
ANIMAL(S): B6C3F1 female mice bred with fertile males of the same strain.
INTERVENTION(S): Collection of endometrial tissue at necropsy from nonimplanted and implanted sites.
MAIN OUTCOME MEASURE(S): IL-1R, IL-1RN, and MCP-1 mRNA expression by quantitative reverse-transcription polymerase chain reaction and protein expression by enzyme-linked immunosorbent assay and immunohistochemistry.
RESULT(S): The expression of the signaling IL-1R1 significantly increased in the first 2 days of gestation, which corresponded to the inflammatory-like period triggered by the seminal fluid, before increasing again at the implantation window and lasting throughout embryo implantation. The expression of inhibitory IL-1R2 and IL-1RN concomitantly increased during gestational days 1-2 but remained low, particularly within the embryo implantation sites and throughout the implantation period. The expression of MCP-1 significantly increased only at the embryo implantation sites and showed a significant positive correlation with IL-1R1 expression.
CONCLUSION(S): Our data identified for the first time synchronous changes in endometrial IL-1R throughout early gestation in vivo and point to a deep modulation of endometrial receptivity to IL-1 by embryo-driven signals. This may play a key role in the creation of a receptive phenotype in the maternal endometrium and represent a key mechanism underlying embryo implantation.
研究白细胞介素-1 受体 (IL-1R)、受体拮抗剂 (IL-1RN) 和单核细胞趋化蛋白 1 (MCP-1) 在小鼠早孕期间的表达动力学。
评估整个早孕期间的 IL-1R、IL-1RN 和 MCP-1。
生殖实验室。
B6C3F1 雌性小鼠与同品系的可育雄性交配。
在尸检时从未着床和着床部位采集子宫内膜组织。
通过定量逆转录聚合酶链反应检测 IL-1R、IL-1RN 和 MCP-1 的 mRNA 表达，通过酶联免疫吸附试验和免疫组织化学检测蛋白质表达。
信号传导 IL-1R1 的表达在妊娠的前 2 天显著增加，这与精液触发的炎症样时期相对应，然后在着床窗口再次增加，并持续到胚胎着床。抑制性 IL-1R2 和 IL-1RN 的表达在妊娠第 1-2 天同时增加，但保持较低水平，特别是在胚胎着床部位和整个着床期。MCP-1 的表达仅在胚胎着床部位显著增加，并与 IL-1R1 的表达呈显著正相关。
我们的数据首次在体内鉴定了整个早孕期间子宫内膜 IL-1R 的同步变化，并指出胚胎驱动的信号对子宫内膜 IL-1 受体的深度调节。这可能在母体子宫内膜的接受表型的创造中发挥关键作用，并代表胚胎着床的关键机制。
