Papsidero L D, Poiesz B J, Montagna R A
Cellular Products, Inc., Buffalo, NY.
Hybridoma. 1988 Apr;7(2):117-28. doi: 10.1089/hyb.1988.7.117.
A murine monoclonal antibody (MAb), 10E9, has been generated which identifies a conserved and immunodominant epitope of the human immunodeficiency virus (HIV) transmembrane protein, gp41. The MAb reacts with the protein backbone of the mature env gene product and also with polyprotein precursor, gp160. Human sera were tested for their ability to competitively inhibit the immunoreactivity of MAb 10E9. Of 100 serum samples obtained from patients with acquired immune deficiency syndrome (AIDS) or AIDS-related complex (ARC), all showed strong inhibition to the reaction. In contrast, sera obtained from normal donors or those with other viral infections failed to perturb the binding activity of MAb 10E9. The geographic diversity of the AIDS/ARC patients studied provides evidence that the 10E9 epitope of gp41 is highly conserved.
已产生一种鼠单克隆抗体（MAb）10E9，它可识别人类免疫缺陷病毒（HIV）跨膜蛋白gp41的一个保守且免疫显性表位。该单克隆抗体与成熟env基因产物的蛋白质骨架反应，也与多蛋白前体gp160反应。检测了人血清竞争性抑制单克隆抗体10E9免疫反应性的能力。从获得性免疫缺陷综合征（AIDS）或AIDS相关综合征（ARC）患者中获取的100份血清样本均对该反应表现出强烈抑制。相比之下，从正常供体或患有其他病毒感染的人那里获得的血清未能干扰单克隆抗体10E9的结合活性。所研究的AIDS/ARC患者的地域多样性证明gp41的10E9表位高度保守。
