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本文引用的文献

1
Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T. gondii from an aborted lamb from a chronically infected ewe.得克萨斯州一家农场绵羊的弓形虫流产风暴，以及从一只慢性感染母羊的流产羔羊中分离出鼠毒力非典型基因型 T. gondii。
Vet Parasitol. 2013 Feb 18;192(1-3):129-36. doi: 10.1016/j.vetpar.2012.09.037. Epub 2012 Oct 8.
2
Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii.迈向综合方法检测和鉴定刚地弓形虫的分子特征。
Parasitology. 2010 Jan;137(1):1-11. doi: 10.1017/S0031182009991065. Epub 2009 Sep 21.
3
An innovative survey underlining the significant level of contamination by Toxoplasma gondii of ovine meat consumed in France.一项创新性调查强调了法国消费的羊肉中弓形虫的严重污染水平。
Int J Parasitol. 2010 Feb;40(2):193-200. doi: 10.1016/j.ijpara.2009.06.009. Epub 2009 Jul 23.
4
High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA.从美国供人类食用的羔羊中分离出的弓形虫具有高流行率和丰富的非典型基因型。
Int J Parasitol. 2008 Jul;38(8-9):999-1006. doi: 10.1016/j.ijpara.2007.11.012. Epub 2007 Dec 8.
5
Toxoplasma gondii infection in sheep from Haute-Vienne, France: seroprevalence and isolate genotyping by microsatellite analysis.法国上维埃纳省绵羊的弓形虫感染：血清阳性率及通过微卫星分析进行分离株基因分型
Vet Parasitol. 2006 Dec 20;142(3-4):376-9. doi: 10.1016/j.vetpar.2006.07.005. Epub 2006 Aug 21.
6
Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy.意大利撒丁岛采用间接荧光抗体试验（IFAT）和聚合酶链式反应（PCR）检测绵羊和山羊弓形虫病的调查
Vet Parasitol. 2003 Nov 3;117(1-2):15-21. doi: 10.1016/j.vetpar.2003.07.012.
7
Toxoplasma gondii: perfecting an intracellular life style.刚地弓形虫：完善一种细胞内生活方式。
Traffic. 2003 Sep;4(9):581-6. doi: 10.1034/j.1600-0854.2003.00117.x.
8
Non-lethal infection parameters in mice separate sheep Type II Toxoplasma gondii isolates by virulence.小鼠中的非致死性感染参数可根据毒力区分绵羊II型弓形虫分离株。
Comp Immunol Microbiol Infect Dis. 2002 May;25(3):187-95. doi: 10.1016/s0147-9571(01)00039-x.
9
Single tube nested PCR for the detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes.用于检测自然流产母羊胎儿组织中弓形虫的单管巢式聚合酶链反应
Vet Parasitol. 2001 Dec 3;102(1-2):17-27. doi: 10.1016/s0304-4017(01)00526-x.
10
Genotyping of Toxoplasma gondii associated with abortion in sheep.与绵羊流产相关的弓形虫基因分型
J Parasitol. 1999 Apr;85(2):382-4.

意大利撒丁岛绵羊流产中弓形虫II型的分子特征分析

Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy.

作者信息

Chessa Giovanna, Chisu Valentina, Porcu Rosaura, Masala Giovanna

机构信息

Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi, 8, 07100 Sassari, Italy.

出版信息

Parasite. 2014;21:6. doi: 10.1051/parasite/2014007. Epub 2014 Feb 19.

DOI:10.1051/parasite/2014007
PMID:24534616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3927306/
Abstract

During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.

摘要

2009年至2010年期间，对来自意大利撒丁岛流产绵羊胎儿的161份组织样本（142份胎盘、16份大脑和3份肝脏）进行了弓形虫病检测。通过针对刚地弓形虫ITS1区域的巢式聚合酶链反应（PCR）显示阳性结果的器官，还用11个基因标记（SAG1、5'-SAG2、3'-SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）进行了扩增，然后进行PCR/RFLP基因分型。通过PCR分析，在5份胎盘、14份大脑和2份肝脏中发现了刚地弓形虫DNA，所有分离株在所有11个位点的所有11个标记上均显示II型等位基因。结果表明，II型刚地弓形虫与绵羊流产有关。