意大利撒丁岛绵羊流产中弓形虫II型的分子特征分析
Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy.
作者信息
Chessa Giovanna, Chisu Valentina, Porcu Rosaura, Masala Giovanna
机构信息
Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi, 8, 07100 Sassari, Italy.
出版信息
Parasite. 2014;21:6. doi: 10.1051/parasite/2014007. Epub 2014 Feb 19.
Abstract
During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.
摘要
2009年至2010年期间，对来自意大利撒丁岛流产绵羊胎儿的161份组织样本（142份胎盘、16份大脑和3份肝脏）进行了弓形虫病检测。通过针对刚地弓形虫ITS1区域的巢式聚合酶链反应（PCR）显示阳性结果的器官，还用11个基因标记（SAG1、5'-SAG2、3'-SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）进行了扩增，然后进行PCR/RFLP基因分型。通过PCR分析，在5份胎盘、14份大脑和2份肝脏中发现了刚地弓形虫DNA，所有分离株在所有11个位点的所有11个标记上均显示II型等位基因。结果表明，II型刚地弓形虫与绵羊流产有关。
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