Piccinini L A, Goldsmith N K, Schachter B S, Davies T F
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.
J Clin Endocrinol Metab. 1988 Jun;66(6):1307-15. doi: 10.1210/jcem-66-6-1307.
We studied the cellular location of HLA-DR alpha-chain synthesis within the human thyroid gland using the technique of in situ hybridization. Tritium-labeled cRNA probes for the HLA-DR alpha-chain DNA sequence revealed significant HLA-DR alpha-chain gene expression in thyroid glands from patients with Graves' hyperthyroidism and Hashimoto's thyroiditis. Hybridization signals, which were RNA strand specific, were widely distributed over thyroid follicular epithelial cells as well as over areas of lymphoid infiltration, with the highest concentration of HLA-DR alpha-chain mRNA within epithelial cells in Graves' disease follicles adjacent to areas of lymphoid infiltration. Qualitatively similar results were obtained with immunoperoxidase staining of thyroid sections for DR antigen. Thyroid tissue obtained from patients who did not have autoimmune thyroid disease contained no detectable HLA-DR antigen, but in situ hybridization revealed variable levels of HLA-DR alpha-chain mRNA in thyroid epithelial cells in such glands. Some glands had no detectable HLA-DR alpha-chain mRNA levels above background, whereas other adult glands had significant DR-specific mRNA levels. Fetal thyroid tissue, however, was negative for strand-specific HLA-DR alpha-chain transcripts as well as for HLA-DR antigen. These results indicate that thyroid epithelial cells are capable of synthesizing HLA class II antigens. The DR genes were expressed to the highest degree within the thyroid glands of patients with autoimmune thyroid disease, where expression was strongly associated with lymphoid infiltration.
我们采用原位杂交技术研究了人甲状腺内HLA - DRα链合成的细胞定位。针对HLA - DRα链DNA序列的氚标记cRNA探针显示，格雷夫斯氏甲状腺功能亢进症和桥本氏甲状腺炎患者的甲状腺中存在显著的HLA - DRα链基因表达。具有RNA链特异性的杂交信号广泛分布于甲状腺滤泡上皮细胞以及淋巴浸润区域，在格雷夫斯病滤泡中与淋巴浸润区域相邻的上皮细胞内，HLA - DRα链mRNA浓度最高。对甲状腺切片进行DR抗原的免疫过氧化物酶染色获得了定性相似的结果。取自无自身免疫性甲状腺疾病患者的甲状腺组织未检测到可检测的HLA - DR抗原，但原位杂交显示此类腺体的甲状腺上皮细胞中HLA - DRα链mRNA水平存在差异。一些腺体未检测到高于背景水平的HLA - DRα链mRNA，而其他成年腺体则有显著的DR特异性mRNA水平。然而，胎儿甲状腺组织中链特异性HLA - DRα链转录本以及HLA - DR抗原均为阴性。这些结果表明甲状腺上皮细胞能够合成HLA II类抗原。DR基因在自身免疫性甲状腺疾病患者的甲状腺中表达程度最高，且其表达与淋巴浸润密切相关。
