Ren Wen, Chen Feng, Zhang Yi-fei, Zhang Qian, Wang Xiao-yan, Liu Ying-yi, Yuan Chong-yang, Ma Qing-wei, Xu Tao, Zheng Shu-guo
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2014 Feb 18;46(1):25-9.
To identify Streptococcus mutans (S. mutans) in carious patients' saliva using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and to establish a faster and more accurate method to identify S. mutans.
In this study, a total of 90 carious patients from Department of Endodontics of Peking University School of Stomatology were recruited. All these patients' saliva was collected. After extracting the protein of the samples, they were identified by MALDI-TOF MS. The composite profile was analyzed using BioExplorer 1.0 software. The scores ≥ 25 were considered as S. mutans, whereas the scores <25 were as considered as non S. mutans. Finally, these results were compared with 16S rDNA sequencing to figure out the sensitivity and concordance rate, respectively.
The sensitivity of MALDI-TOF MS was 96.0%, and the concordance rate compared with 16S rDNA sequencing was as high as 98.7%.
MALDI-TOF MS is high throughput, rapid and easy to perform in comparison to other conventional methods. It has a high sensivity and concordance rate. Thus, MALDI-TOF MS can serve as an effective tool for identification of S. mutans.
运用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）鉴定龋病患者唾液中的变形链球菌（S. mutans），并建立一种更快、更准确的变形链球菌鉴定方法。
本研究招募了北京大学口腔医学院牙体牙髓科的90例龋病患者，收集所有患者的唾液。提取样本蛋白质后，采用MALDI-TOF MS进行鉴定，使用BioExplorer 1.0软件分析综合图谱。得分≥25者判定为变形链球菌，得分<25者判定为非变形链球菌。最后，将这些结果与16S rDNA测序结果进行比较，分别计算灵敏度和符合率。
MALDI-TOF MS的灵敏度为96.0%，与16S rDNA测序的符合率高达98.7%。
与其他传统方法相比，MALDI-TOF MS具有高通量、快速且易于操作的特点，具有较高的灵敏度和符合率。因此，MALDI-TOF MS可作为鉴定变形链球菌的有效工具。
