一名患有瘤型麻风病且伴有抗细胞骨架抗体的患者。

A patient with lepromatous leprosy and anticytoskeletal antibodies.

作者信息

Frey F L, Gottlieb A B, Levis W R

机构信息

Department of Immunology and Dermatology, Rockefeller University, New York, NY 10021-6399.

出版信息

J Am Acad Dermatol. 1988 May;18(5 Pt 2):1179-84. doi: 10.1016/s0190-9622(88)70120-6.

DOI:10.1016/s0190-9622(88)70120-6

PMID:2453540

Abstract

Sera from 34 patients with lepromatous leprosy were screened for the presence of autoantibodies by indirect immunofluorescence using two epithelial cell lines, PTK2 and HEp2, as substrates. Indirect immunofluorescence staining of both substrates with the serum of a patient with lepromatous leprosy revealed a cytoplasmic intermediate filament staining pattern. After exposure of PTK2 cells to colchicine, the filaments collapsed into thick perinuclear coils, confirming the presence of intermediate filament reactivity. Immunofluorescence of rat fibroblasts with the same serum also revealed an intermediate filamentous staining pattern. Human keratinocytes exposed to the patient's serum revealed a diffuse cytoplasmic staining pattern. Our study suggests the presence of autoantibodies to cytoskeletal intermediate filaments or to molecules associated with vimentin and possibly keratin subunit proteins in the serum of a patient with lepromatous leprosy.

摘要

使用两种上皮细胞系PTK2和HEp2作为底物，通过间接免疫荧光法对34例瘤型麻风患者的血清进行自身抗体筛查。用瘤型麻风患者的血清对两种底物进行间接免疫荧光染色，结果显示为细胞质中间丝染色模式。将PTK2细胞暴露于秋水仙碱后，细丝聚集成粗大的核周线圈，证实存在中间丝反应性。用同一血清对大鼠成纤维细胞进行免疫荧光检测，也显示出中间丝染色模式。暴露于患者血清中的人角质形成细胞显示出弥漫性细胞质染色模式。我们的研究表明，瘤型麻风患者血清中存在针对细胞骨架中间丝或与波形蛋白以及可能的角蛋白亚基蛋白相关分子的自身抗体。