Parsons R L, Neel D S
Department of Anatomy and Neurobiology, College of Medicine, University of Vermont, Burlington 05405.
J Auton Nerv Syst. 1987 Dec;21(2-3):135-43. doi: 10.1016/0165-1838(87)90016-6.
An immunohistochemical study was undertaken to determine the distribution of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers in the cardiac septum of the mudpuppy, Necturus maculosus. Numerous long, CGRP-immunoreactive nerve fibers course across the septum, run in the nerve trunks connecting clusters of postganglionic parasympathetic cells, form complexes over groups of ganglion cells and make pericellular networks around individual ganglion cells. The postganglionic parasympathetic neurons and small intensely fluorescent (SIF)-like cells did not exhibit CGRP immunoreactivity. Most of the CGRP-immunoreactive nerve fibers also are labeled for substance P. In freshly dissected preparations, the staining pattern for CGRP was not similar to that obtained using an antiserum against synaptic vesicle membrane, which appears to preferentially label cholinergic preganglionic terminals on all postganglionic parasympathetic cells in the mudpuppy preparation. Further, in explanted ganglia (maintained 10 days in culture) almost no reactivity was obtained with the antivesicle antiserum whereas numerous nerve fibers still exhibited CGRP-immunoreactivity. These observations demonstrate that the CGRP-immunoreactive nerve fibers are not parasympathetic preganglionic axons. Rather we suggest that the CGRP-immunoreactive nerve fibers are processes of primary sensory fibers.
进行了一项免疫组织化学研究，以确定泥螈（Necturus maculosus）心脏隔膜中降钙素基因相关肽（CGRP）免疫反应性神经纤维的分布。许多长的CGRP免疫反应性神经纤维横穿隔膜，在连接节后副交感神经细胞簇的神经干中运行，在神经节细胞群上形成复合体，并在单个神经节细胞周围形成细胞周网络。节后副交感神经元和小而强荧光（SIF）样细胞未表现出CGRP免疫反应性。大多数CGRP免疫反应性神经纤维也被P物质标记。在新鲜解剖的标本中，CGRP的染色模式与使用抗突触小泡膜抗血清获得的染色模式不同，后者似乎优先标记泥螈标本中所有节后副交感细胞上的胆碱能节前终末。此外，在体外培养10天的神经节中，抗小泡抗血清几乎没有反应，而许多神经纤维仍表现出CGRP免疫反应性。这些观察结果表明，CGRP免疫反应性神经纤维不是副交感节前轴突。相反，我们认为CGRP免疫反应性神经纤维是初级感觉纤维的突起。
